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Trap column-based intact mass spectrometry for rapid and accurate evaluation of protein molecular weight
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Gyu-Min | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Jeong, Hee-Jin | - |
dc.date.accessioned | 2022-09-30T05:42:47Z | - |
dc.date.available | 2022-09-30T05:42:47Z | - |
dc.date.created | 2022-06-09 | - |
dc.date.issued | 2022-05 | - |
dc.identifier.citation | RSC Advances, Vol.12 No.25, pp.15643-15651 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | https://hdl.handle.net/10371/184801 | - |
dc.description.abstract | The determination of the molecular weight (MW) of a protein using high-resolution mass spectrometry (MS) is a crucial tool used to confirm whether the protein was correctly expressed and adequately purified. However, a non-volatile buffer is normally used for protein purification and storage. Therefore, a pre-treatment step using ultrafiltration (UF) is required to exchange the buffer with a volatile buffer prior to the introduction of the protein sample into the MS equipment. This pre-treatment step is time-consuming. In this study, a trap column-based pre-treatment method applied in a nano-LC system was developed for rapid and convenient analysis of the MW of proteins. First, the trap column system was compared with the conventional UF treatment system and non-treatment system using bovine serum albumin. Subsequently, the trap column system was applied to analyze the MW of commercially available and lab-synthesized recombinant proteins. The intensity of the base peak and signal-to-noise ratio of the trap column-based pre-treated protein were higher than those of the UF-treated protein. Moreover, the entire automated procedure of the trap column-based system was conducted within 20 min, which confirms its use in versatile and accurate protein identification. | - |
dc.language | 영어 | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Trap column-based intact mass spectrometry for rapid and accurate evaluation of protein molecular weight | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/d2ra00429a | - |
dc.citation.journaltitle | RSC Advances | - |
dc.identifier.wosid | 000799086500001 | - |
dc.identifier.scopusid | 2-s2.0-85131666487 | - |
dc.citation.endpage | 15651 | - |
dc.citation.number | 25 | - |
dc.citation.startpage | 15643 | - |
dc.citation.volume | 12 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Gee | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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