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Establishment of a piglet model for peritoneal metastasis of ovarian cancer
DC Field | Value | Language |
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dc.contributor.author | Lee, Eun Ji | - |
dc.contributor.author | Park, Soo Jin | - |
dc.contributor.author | Seol, Aeran | - |
dc.contributor.author | Lim, Hyunji | - |
dc.contributor.author | Park, Sumin | - |
dc.contributor.author | Ahn, Ji Yeon | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.contributor.author | Kim, Hee Seung | - |
dc.contributor.author | Park, Ji Won | - |
dc.contributor.author | Song, Gwonhwa | - |
dc.contributor.author | Ham, Jiyen | - |
dc.contributor.author | Park, Sunwoo | - |
dc.contributor.author | Yim, Ga Won | - |
dc.contributor.author | Shim, Seung-Hyuk | - |
dc.contributor.author | Kang, Beong-Cheol | - |
dc.contributor.author | Chang, Suk Joon | - |
dc.contributor.author | Lim, Whasun | - |
dc.contributor.author | Lee, Jung Chan | - |
dc.date.accessioned | 2022-10-04T08:28:22Z | - |
dc.date.available | 2022-10-04T08:28:22Z | - |
dc.date.created | 2022-07-29 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.citation | Journal of Translational Medicine, Vol.20 No.1, p. 329 | - |
dc.identifier.issn | 1479-5876 | - |
dc.identifier.uri | https://hdl.handle.net/10371/185237 | - |
dc.description.abstract | © 2022, The Author(s).Background: A piglet model for peritoneal metastasis (PM) of ovarian cancer was developed. It will contribute to establishing innovative chemotherapeutical and surgical strategies without any limitation on rodent models. Methods: A total of 12 four- to five-week-old piglets of 7 to 8 kg were used. Two phases of ovarian cancer cell injections were performed with laparoscopic surgery. In phase I trial, 5.0 × 106 SK-OV-3 cells in 0.1 ml suspension were inoculated into the omentum, peritoneum, and uterine horns of two piglets twice with a one-week interval. In the phase II trial, 5.0 × 106 SNU-008 cells in 0.1 ml suspension were injected only into uterine horns within the same time frame because tumor implantation after inoculation of SK-OV-3 cells was not observed at the omentum or peritoneum in the phase I trial. Modified peritoneal cancer index (PCI) score was used to monitor tumorigenesis up to 4 weeks after inoculation. Tumor tissues disseminated in the peritoneum 4 weeks after injection were used for histological examination with hematoxylin and eosin (H&E) and paired-box gene 8 (PAX-8) staining. Results: In the phase I trial, two piglets showed PM with modified PCI scores of 5 and 4 at 3 weeks after the first inoculation, which increased to 14 and 15 after 4 weeks, respectively. In the phase II trial, PM was detected in eight of ten piglets, which showed modified PCI scores of 6 to 12 at 4 weeks after the first inoculation. The overall incidence of PM from the total of 12 piglets after inoculation was 75%. Immunohistochemical H&E and PAX-8 staining confirmed metastatic tumors. Conclusions: This study provides strong evidence that piglets can be employed as a model for PM by inoculating ovarian cancer cell lines from humans. Using two cell lines, the PM rate is 75%. | - |
dc.language | 영어 | - |
dc.publisher | BioMed Central | - |
dc.title | Establishment of a piglet model for peritoneal metastasis of ovarian cancer | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s12967-022-03533-1 | - |
dc.citation.journaltitle | Journal of Translational Medicine | - |
dc.identifier.wosid | 000828689900001 | - |
dc.identifier.scopusid | 2-s2.0-85134507493 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 329 | - |
dc.citation.volume | 20 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lim, Jeong Mook | - |
dc.contributor.affiliatedAuthor | Kim, Hee Seung | - |
dc.contributor.affiliatedAuthor | Lee, Jung Chan | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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