Development of a Rapid Fluorescent Diagnostic System for Early Detection of the Highly Pathogenic Avian Influenza H5 Clade Viruses in Chicken Stool

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Duong, Bao-Tuan; Than, Duc-Duong; Ju, Bae-Gum; Trinh, Thuy-Tien Thi; Mok, Chris-Ka Pun; Jeong, Ju-Hwan; Song, Min-Suk; Baek, Yun-Hee; Park, Hyun; Yeo, Seon-Ju

Issue Date
Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, Vol.23 No.11, p. 6301
Rapid diagnosis is essential for the control and prevention of H5 highly pathogenic avian influenza viruses (HPAIVs). However, highly sensitive and rapid diagnostic systems have shown limited performance due to specific antibody scarcity. In this study, two novel specific monoclonal antibodies (mAbs) for clade H5Nx viruses were developed by using an immunogen from a reversed genetic influenza virus (RGV). These mAbs were combined with fluorescence europium nanoparticles and an optimized lysis buffer, which were further used for developing a fluorescent immunochromatographic rapid strip test (FICT) for early detection of H5Nx influenza viruses on chicken stool samples. The result indicates that the limit of detection (LoD) of the developed FICT was 40 HAU/mL for detection of HPAIV H5 clade in spiked chicken stool samples, which corresponded to 4.78 x 10(4) RNA copies as obtained from real-time polymerase chain reaction (RT-PCR). An experimental challenge of chicken with H5N6 HPAIV is lethal for chicken three days post-infection (DPI). Interestingly, our FICT could detect H5N6 in stool samples at 2 DPI earlier, with 100% relative sensitivity in comparison with RT-PCR, and it showed 50% higher sensitivity than the traditional colloidal gold-based rapid diagnostic test using the same mAbs pair. In conclusion, our rapid diagnostic method can be utilized for the early detection of H5Nx HPAIVs in avian fecal samples from poultry farms or for influenza surveillance in wild migratory birds.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biomedical Sciences (대학원 의과학과)Journal Papers (저널논문_의과학과)
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