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Constructing multi-enzymatic cascade reactions for selective production of 6-bromoindirubin from tryptophan in Escherichia coli

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dc.contributor.authorLee, Jeongchan-
dc.contributor.authorKim, Joonwon-
dc.contributor.authorKim, Hyun-
dc.contributor.authorPark, Hyun A.-
dc.contributor.authorKim, Jin Young-
dc.contributor.authorKim, Eun-Jung-
dc.contributor.authorYang, Yung-Hun-
dc.contributor.authorChoi, Kwon-Young-
dc.contributor.authorKim, Byung-Gee-
dc.date.accessioned2022-10-07T01:27:34Z-
dc.date.available2022-10-07T01:27:34Z-
dc.date.created2022-09-20-
dc.date.issued2022-10-
dc.identifier.citationBiotechnology and Bioengineering, Vol.119 No.10, pp.2938-2949-
dc.identifier.issn0006-3592-
dc.identifier.urihttps://hdl.handle.net/10371/185577-
dc.description.abstract6-Bromoindirubin (6BrIR), found in Murex sea snails, is a precursor of indirubin-derivatives anticancer drugs. However, its synthesis remains limited due to uncharacterized biosynthetic pathways and difficulties in site-specific bromination and oxidation at the indole ring. Here, we present an efficient 6BrIR production strategy in Escherichia coli by using four enzymes, that is, tryptophan 6-halogenase fused with flavin reductase Fre (Fre-L3-SttH), tryptophanase (TnaA), toluene 4-monooxygenase (PmT4MO), and flavin-containing monooxygenase (MaFMO). Although most indole oxygenases preferentially oxygenate the electronically active C3 position of indole, PmT4MO was newly characterized to perform C2 oxygenation of 6-bromoindole with 45% yield to produce 6-bromo-2-oxindole. In addition, 6BrIR was selectively generated without indigo and indirubin byproducts by controlling the reducing power of cysteine and oxygen supply during the MaFMO reaction. These approaches led to 34.1 mg/L 6BrIR productions, making it possible to produce the critical precursor of the anticancer drugs only from natural ingredients such as tryptophan, NaBr, and oxygen.-
dc.language영어-
dc.publisherWiley - V C H Verlag GmbbH & Co.-
dc.titleConstructing multi-enzymatic cascade reactions for selective production of 6-bromoindirubin from tryptophan in Escherichia coli-
dc.typeArticle-
dc.identifier.doi10.1002/bit.28188-
dc.citation.journaltitleBiotechnology and Bioengineering-
dc.identifier.wosid000850543800026-
dc.identifier.scopusid2-s2.0-85135502433-
dc.citation.endpage2949-
dc.citation.number10-
dc.citation.startpage2938-
dc.citation.volume119-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Byung-Gee-
dc.type.docTypeArticle-
dc.description.journalClass1-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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