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Enzymatic synthesis of puerarin glucosides using Leuconostoc Dextransucrase
Cited 13 time in
Web of Science
Cited 16 time in Scopus
- Authors
- Issue Date
- 2012-09
- Publisher
- 한국미생물·생명공학회
- Citation
- Journal of Microbiology and Biotechnology, Vol.22 No.9, pp.1224-1229
- Abstract
- Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as α-D-glucosyl- (1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials. © The Korean Society for Microbiology and Biotechnology.
- ISSN
- 1017-7825
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