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Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system

Cited 4 time in Web of Science Cited 4 time in Scopus
Authors

Ko, Jin-A; Jeong, Hyung Jae; Ryu, Young Bae; Park, Su-Jin; Wee, Young-Jung; Kim, Doman; Kim, Young-Min; Lee, Woo Song

Issue Date
2012-04
Publisher
한국미생물·생명공학회
Citation
Journal of Microbiology and Biotechnology, Vol.22 No.4, pp.510-515
Abstract
A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction (OD 600) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of 17°C. These results may effectively apply to the heterologous expression of other large DexT genes. © The Korean Society for Microbiology and Biotechnology.
ISSN
1017-7825
URI
https://hdl.handle.net/10371/186431
DOI
https://doi.org/10.4014/jmb.1111.11032
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