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Transglutaminase 2 mediates transcriptional regulation through BAF250a polyamination
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyo-Jun | - |
dc.contributor.author | Lee, Jin-Haeng | - |
dc.contributor.author | Cho, Sung Yup | - |
dc.contributor.author | Jeon, Ju Hong | - |
dc.contributor.author | Kim, In Gyu | - |
dc.date.accessioned | 2023-04-18T06:22:34Z | - |
dc.date.available | 2023-04-18T06:22:34Z | - |
dc.date.created | 2021-05-27 | - |
dc.date.created | 2021-05-27 | - |
dc.date.created | 2021-05-27 | - |
dc.date.created | 2021-05-27 | - |
dc.date.issued | 2021-04 | - |
dc.identifier.citation | Genes & Genomics, Vol.43 No.4, pp.333-342 | - |
dc.identifier.issn | 1976-9571 | - |
dc.identifier.uri | https://hdl.handle.net/10371/190055 | - |
dc.description.abstract | Background Transglutaminase 2 (TG2) mediates protein modifications by crosslinking or by incorporating polyamine in response to oxidative or DNA-damaging stress, thereby regulating apoptosis, extracellular matrix formation, and inflammation. The regulation of transcriptional activity by TG2-mediated histone serotonylation or by Sp1 crosslinking may also contribute to cellular stress responses. Objective In this study, we attempted to identify TG2-interacting proteins to better understand the role of TG2 in transcriptional regulation. Methods Using a yeast two-hybrid assay to screen a HeLa cell cDNA library, we found that TG2 bound BAF250a, a core subunit of the cBAF chromatin remodeling complex, through an interaction between the TG2 barrel 1 and BAF250a C-terminal domains. Results TG2 was pulled down with a GST-BAF250a C-term fusion protein. Moreover, TG2 and BAF250a were co-fractionated using P11 chromatography, and co-immunoprecipitated. A transamidation reaction showed that TG2 mediated incorporation of polyamine into BAF250a. In glucocorticoid response-element reporter-expressing cells, TG2 overexpression increased the luciferase reporter activity in a transamidation-dependent manner. In addition, a comparison of genome-wide gene expression between wild-type and TG2-deficient primary hepatocytes in response to dexamethasone treatment showed that TG2 further enhanced or suppressed the expression of dexamethasone-regulated genes that were identified by a gene ontology enrichment analysis. Conclusion Thus, our results indicate that TG2 regulates transcriptional activity through BAF250a polyamination. | - |
dc.language | 영어 | - |
dc.publisher | 한국유전학회 | - |
dc.title | Transglutaminase 2 mediates transcriptional regulation through BAF250a polyamination | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s13258-021-01055-6 | - |
dc.citation.journaltitle | Genes & Genomics | - |
dc.identifier.wosid | 000616049400002 | - |
dc.identifier.scopusid | 2-s2.0-85100679938 | - |
dc.citation.endpage | 342 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 333 | - |
dc.citation.volume | 43 | - |
dc.identifier.kciid | ART002707618 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Cho, Sung Yup | - |
dc.contributor.affiliatedAuthor | Jeon, Ju Hong | - |
dc.contributor.affiliatedAuthor | Kim, In Gyu | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordAuthor | Transglutaminase 2 (TG2) | - |
dc.subject.keywordAuthor | BAF250a | - |
dc.subject.keywordAuthor | Transamidation | - |
dc.subject.keywordAuthor | Dexamethasone | - |
dc.subject.keywordAuthor | Transcriptional activity | - |
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