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The structure of AcrIE4-F7 reveals a common strategy for dual CRISPR inhibition by targeting PAM recognition sites
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, Sung-Hyun | - |
dc.contributor.author | Lee, Gyujin | - |
dc.contributor.author | Park, Changkon | - |
dc.contributor.author | Koo, Jasung | - |
dc.contributor.author | Kim, Eun-Hee | - |
dc.contributor.author | Bae, Euiyoung | - |
dc.contributor.author | Suh, Jeong-Yong | - |
dc.date.accessioned | 2023-04-19T00:20:49Z | - |
dc.date.available | 2023-04-19T00:20:49Z | - |
dc.date.created | 2022-04-20 | - |
dc.date.created | 2022-04-20 | - |
dc.date.issued | 2022-02 | - |
dc.identifier.citation | Nucleic Acids Research, Vol.50 No.4, pp.2363-2376 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://hdl.handle.net/10371/190190 | - |
dc.description.abstract | © 2022 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research.Bacteria and archaea use the CRISPR-Cas system to fend off invasions of bacteriophages and foreign plasmids. In response, bacteriophages encode anti-CRISPR (Acr) proteins that potently inhibit host Cas proteins to suppress CRISPR-mediated immunity. AcrIE4-F7, which was isolated from Pseudomonas citronellolis, is a fused form of AcrIE4 and AcrIF7 that inhibits both type I-E and type I-F CRISPR-Cas systems. Here, we determined the structure of AcrIE4-F7 and identified its Cas target proteins. The N-terminal AcrIE4 domain adopts a novel α-helical fold that targets the PAM interaction site of the type I-E Cas8e subunit. The C-terminal AcrIF7 domain exhibits an αβ fold like native AcrIF7, which disables target DNA recognition by the PAM interaction site in the type I-F Cas8f subunit. The two Acr domains are connected by a flexible linker that allows prompt docking onto their cognate Cas8 targets. Conserved negative charges in each Acr domain are required for interaction with their Cas8 targets. Our results illustrate a common mechanism by which AcrIE4-F7 inhibits divergent CRISPR-Cas types. | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | The structure of AcrIE4-F7 reveals a common strategy for dual CRISPR inhibition by targeting PAM recognition sites | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/nar/gkac096 | - |
dc.citation.journaltitle | Nucleic Acids Research | - |
dc.identifier.wosid | 000764490000001 | - |
dc.identifier.scopusid | 2-s2.0-85125549992 | - |
dc.citation.endpage | 2376 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 2363 | - |
dc.citation.volume | 50 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Bae, Euiyoung | - |
dc.contributor.affiliatedAuthor | Suh, Jeong-Yong | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | EVOLUTIONARY CLASSIFICATION | - |
dc.subject.keywordPlus | WEB SERVER | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | CONSERVATION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | ANGLES | - |
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