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Characterization and complete genome sequence of the Shigella bacteriophage pSf-1
Cited 36 time in
Web of Science
Cited 41 time in Scopus
- Authors
- Issue Date
- 2013-12
- Publisher
- Elsevier BV
- Citation
- Research in Microbiology, Vol.164 No.10, pp.979-986
- Abstract
- Shigellosis is a global health problem, and Shigella flexneri is the major cause of this disease. In this study, we isolated a virulent Siphoviridae bacteriophage (phage), pSf-1, that infects S. flexneri. This phage was isolated from the Han River in Korea and was found to infect S. flexneri, Shigella boydii, and Shigella sonnei. One-step growth analysis revealed that this phage has a short latent period (10 mm) and a large burst size (86.86 PFU/cell), indicating that pSf-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSf-1 is composed of 51,821 bp with a G C content of 44.02%. The genome encodes 94 putative ORFs, 71 putative promoters, and 60 transcriptional terminator regions. Genome sequence analysis of pSf-1 and comparative analysis with the homologous Shigella phage Shfl1 revealed that there is a high degree of similarity between pSf-1 and Shfl1 in 54 of the 94 ORFs of pSf-1. The results of this investigation indicate that pSf-1 is a novel Shigella phage and that this phage might have potential uses against shigellosis. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
- ISSN
- 0923-2508
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