Publications
Detailed Information
Computer-based engineering of thermostabilized antibody fragments
Cited 10 time in
Web of Science
Cited 12 time in Scopus
- Authors
- Issue Date
- 2020-03
- Publisher
- American Institute of Chemical Engineers
- Citation
- AICHE Journal, Vol.66 No.3, p. e16864
- Abstract
- We used the molecular modeling program Rosetta to identify clusters of amino acid substitutions in antibody fragments (scFvs and scAbs) that improve global protein stability and resistance to thermal deactivation. Using this methodology, we increased the melting temperature (T-m) and resistance to heat treatment of an antibody fragment that binds to the Clostridium botulinum hemagglutinin protein (anti-HA33). Two designed antibody fragment variants with two amino acid replacement clusters, designed to stabilize local regions, were shown to have both higher T-m compared to the parental scFv and importantly to retain full antigen binding activity after 2 hr of incubation at 70 degrees C. The crystal structure of one thermostabilized scFv variants was solved at 1.6 angstrom and shown to be in close agreement with the RosettaAntibody model prediction.
- ISSN
- 0001-1541
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.