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Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2,3-butanediol dehydrogenase and expression of NADH oxidase
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, Sang-Jeong | - |
dc.contributor.author | Kim, Sujin | - |
dc.contributor.author | Hahn, Ji-Sook | - |
dc.date.accessioned | 2023-04-19T08:47:13Z | - |
dc.date.available | 2023-04-19T08:47:13Z | - |
dc.date.created | 2018-06-29 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.citation | Scientific Reports, Vol.6, p. 27667 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191170 | - |
dc.description.abstract | Acetoin is widely used in food and cosmetic industry as taste and fragrance enhancer. For acetoin production in this study, Saccharomyces cerevisiae JHY605 was used as a host strain, where the production of ethanol and glycerol was largely eliminated by deleting five alcohol dehydrogenase genes (ADH1, ADH2, ADH3, ADH4, and ADH5) and two glycerol 3-phosphate dehydrogenase genes (GPD1 and GPD2). To improve acetoin production, acetoin biosynthetic genes from Bacillus subtilis encoding alpha-acetolactate synthase (AlsS) and alpha-acetolactate decarboxylase (AlsD) were overexpressed, and BDH1 encoding butanediol dehydrogenase, which converts acetoin to 2,3-butanediol, was deleted. Furthermore, by NAD(+) regeneration through overexpression of water-forming NADH oxidase (NoxE) from Lactococcus lactis, the cofactor imbalance generated during the acetoin production from glucose was successfully relieved. As a result, in fed-batch fermentation, the engineered strain JHY617-SDN produced 100.1 g/L acetoin with a yield of 0.44 g/g glucose. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2,3-butanediol dehydrogenase and expression of NADH oxidase | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/srep27667 | - |
dc.citation.journaltitle | Scientific Reports | - |
dc.identifier.wosid | 000377365500001 | - |
dc.identifier.scopusid | 2-s2.0-84974601942 | - |
dc.citation.startpage | 27667 | - |
dc.citation.volume | 6 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Hahn, Ji-Sook | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SERRATIA-MARCESCENS | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ENTEROBACTER | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordPlus | GPD1 | - |
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