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Giant Y79 retinoblastoma cells contain functionally active T-type calcium channels
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sooyun | - |
dc.contributor.author | Guzman, Segundo Jose | - |
dc.contributor.author | Jo, Dong Hyun | - |
dc.contributor.author | Cho, Chang Sik | - |
dc.contributor.author | Kim, Jeong Hun | - |
dc.date.accessioned | 2023-04-25T07:30:23Z | - |
dc.date.available | 2023-04-25T07:30:23Z | - |
dc.date.created | 2021-08-27 | - |
dc.date.created | 2021-08-27 | - |
dc.date.created | 2021-08-27 | - |
dc.date.created | 2021-08-27 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.citation | Pflugers Archiv European Journal of Physiology, Vol.473 No.10, pp.1631-1639 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191474 | - |
dc.description.abstract | Retinoblastoma is the most common malignant intraocular tumor in children. Y79 human retinoblastoma cells are in vitro models of retinal tumors used for drug screening. Undifferentiated Y79 cells originate from a primitive multi-potential neuroectodermal cell and express neuronal and glial properties. However, the nature of cellular heterogeneity in Y79 cells is unclear because functional methods to characterize neurons or glial cells have not been employed to Y79 cells. Here, we perform patch-clamp recordings to characterize electrophysiological properties in retinoblastoma cells. We identified a population of large-sized Y79 cells (i.e., giant cells, similar to 40-mu m diameter), hyperpolarized resting membrane potential (-54 mV), and low input resistance (similar to 600 M Omega), indicating electrically mature cells. We also found that giant Y79 cells contain increased density of T-type calcium channels. Finally, we found that T-type calcium channels are active only in giant cells suggesting that cancer treatments aimed to prevent calcium influx in retinoblastomas should be tested in giant cells. | - |
dc.language | 영어 | - |
dc.publisher | Springer Verlag | - |
dc.title | Giant Y79 retinoblastoma cells contain functionally active T-type calcium channels | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00424-021-02612-4 | - |
dc.citation.journaltitle | Pflugers Archiv European Journal of Physiology | - |
dc.identifier.wosid | 000685149500001 | - |
dc.identifier.scopusid | 2-s2.0-85112430338 | - |
dc.citation.endpage | 1639 | - |
dc.citation.number | 10 | - |
dc.citation.startpage | 1631 | - |
dc.citation.volume | 473 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Jo, Dong Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Jeong Hun | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | TOOLS | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | LINE | - |
dc.subject.keywordAuthor | Retinoblastoma | - |
dc.subject.keywordAuthor | Y79 | - |
dc.subject.keywordAuthor | Cancer | - |
dc.subject.keywordAuthor | Resting membrane potential | - |
dc.subject.keywordAuthor | T-type calcium channel | - |
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