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Bispecific anti-mPDGFR beta x cotinine scFv-C-kappa-scFv fusion protein and cotinine-duocarmycin can form antibody-drug conjugate-like complexes that exert cytotoxicity against mPDGFR beta expressing cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Soohyun | - |
dc.contributor.author | Kim, Hyori | - |
dc.contributor.author | Jo, Dong Hyun | - |
dc.contributor.author | Kim, Jeong Hun | - |
dc.contributor.author | Kim, Su Ree | - |
dc.contributor.author | Kang, Dongmin | - |
dc.contributor.author | Hwang, Dobeen | - |
dc.contributor.author | Chung, Junho | - |
dc.date.accessioned | 2023-04-25T07:31:54Z | - |
dc.date.available | 2023-04-25T07:31:54Z | - |
dc.date.created | 2019-11-29 | - |
dc.date.created | 2019-11-29 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | Methods, Vol.154, pp.125-135 | - |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191507 | - |
dc.description.abstract | Antibody selection for antibody-drug conjugates (ADCs) has traditionally depended on its internalization into the target cell, although ADC efficacy also relies on recycling of the receptor-ADC complex, endo-lysosomal trafficking, and subsequent linker/antibody proteolysis. In this study, we observed that a bispecific anti-murine platelet-derived growth factor receptor beta (mPDGFR beta) x cotinine single-chain variable fragment (scFv)-kappa constant region (C-kappa)-scFv fusion protein and cotinine-duocarmycin can form an ADC-like complex to induce cytotoxicity against rnPDGFR beta expressing cells. Multiple anti-mPDGFR beta antibody candidates can be produced in this bispecific scFv-C-kappa-scFv fusion protein format and tested for their ability to deliver cotinine-conjugated cytotoxic drugs, thus providing an improved approach for antibody selection in ADC development. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | Bispecific anti-mPDGFR beta x cotinine scFv-C-kappa-scFv fusion protein and cotinine-duocarmycin can form antibody-drug conjugate-like complexes that exert cytotoxicity against mPDGFR beta expressing cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ymeth.2018.10.002 | - |
dc.citation.journaltitle | Methods | - |
dc.identifier.wosid | 000457813100015 | - |
dc.identifier.scopusid | 2-s2.0-85054685647 | - |
dc.citation.endpage | 135 | - |
dc.citation.startpage | 125 | - |
dc.citation.volume | 154 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Jo, Dong Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Jeong Hun | - |
dc.contributor.affiliatedAuthor | Chung, Junho | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GROWTH-FACTOR RECEPTOR | - |
dc.subject.keywordPlus | INTRACELLULAR TRAFFICKING | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODY | - |
dc.subject.keywordPlus | PDGF RECEPTORS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | INTERNALIZATION | - |
dc.subject.keywordPlus | AFFINITY | - |
dc.subject.keywordPlus | ENDOCYTOSIS | - |
dc.subject.keywordPlus | DOXORUBICIN | - |
dc.subject.keywordPlus | XENOGRAFTS | - |
dc.subject.keywordAuthor | Antibody-drug conjugate | - |
dc.subject.keywordAuthor | Bispecific antibody | - |
dc.subject.keywordAuthor | Cotinine | - |
dc.subject.keywordAuthor | Duocarmycin | - |
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