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Interleukin-6 Induces S100A9 Expression in Colonic Epithelial Cells through STAT3 Activation in Experimental Ulcerative Colitis
DC Field | Value | Language |
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dc.contributor.author | Lee, Min Jeoung | - |
dc.contributor.author | Lee, Jin-Ku | - |
dc.contributor.author | Choi, Ji Won | - |
dc.contributor.author | Lee, Chang-Seok | - |
dc.contributor.author | Sim, Ji Hyun | - |
dc.contributor.author | Cho, Chung-Hyun | - |
dc.contributor.author | Lee, Kwang-Ho | - |
dc.contributor.author | Cho, Ik-Hyun | - |
dc.contributor.author | Chung, Myung-Hee | - |
dc.contributor.author | Kim, Hang-Rae | - |
dc.contributor.author | Ye, Sang-Kyu | - |
dc.date.accessioned | 2023-04-26T05:07:42Z | - |
dc.date.available | 2023-04-26T05:07:42Z | - |
dc.date.created | 2018-01-10 | - |
dc.date.created | 2018-01-10 | - |
dc.date.created | 2018-01-10 | - |
dc.date.created | 2018-01-10 | - |
dc.date.created | 2018-01-10 | - |
dc.date.created | 2018-01-10 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | PLOS ONE, Vol.7 No.9, p. e38801 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191565 | - |
dc.description.abstract | Background: Intestinal epithelium is essential for maintaining normal intestinal homeostasis; its breakdown leads to chronic inflammatory pathologies, such as inflammatory bowel diseases (IBDs). Although high concentrations of S100A9 protein and interleukin-6 (IL-6) are found in patients with IBD, the expression mechanism of S100A9 in colonic epithelial cells (CECs) remains elusive. We investigated the role of IL-6 in S100A9 expression in CECs using a colitis model. Methods: IL-6 and S100A9 expression, signal transducer and activator of transcription 3 (STAT3) phosphorylation, and infiltration of immune cells were analyzed in mice with dextran sulfate sodium (DSS)-induced colitis. The effects of soluble gp130-Fc protein (sgp130Fc) and S100A9 small interfering (si) RNA (si-S100A9) on DSS-induced colitis were evaluated. The molecular mechanism of S100A9 expression was investigated in an IL-6-treated Caco-2 cell line using chromatin immunoprecipitation assays. Results: IL-6 concentrations increased significantly in the colon tissues of DSS-treated mice. sgp130Fc or si-S100A9 administration to DSS-treated mice reduced granulocyte infiltration in CECs and induced the down-regulation of S100A9 and colitis disease activity. Treatment with STAT3 inhibitors upon IL-6 stimulation in the Caco-2 cell line demonstrated that IL-6 mediated S100A9 expression through STAT3 activation. Moreover, we found that phospho-STAT3 binds directly to the S100A9 promoter. S100A9 may recruit immune cells into inflamed colon tissues. Conclusions: Elevated S100A9 expression in CECs mediated by an IL-6/STAT3 signaling cascade may play an important role in the development of colitis. | - |
dc.language | 영어 | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Interleukin-6 Induces S100A9 Expression in Colonic Epithelial Cells through STAT3 Activation in Experimental Ulcerative Colitis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0038801 | - |
dc.citation.journaltitle | PLOS ONE | - |
dc.identifier.wosid | 000308577600001 | - |
dc.identifier.scopusid | 2-s2.0-84866039292 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | e38801 | - |
dc.citation.volume | 7 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Lee, Jin-Ku | - |
dc.contributor.affiliatedAuthor | Cho, Chung-Hyun | - |
dc.contributor.affiliatedAuthor | Chung, Myung-Hee | - |
dc.contributor.affiliatedAuthor | Kim, Hang-Rae | - |
dc.contributor.affiliatedAuthor | Ye, Sang-Kyu | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | INFLAMMATORY-BOWEL-DISEASE | - |
dc.subject.keywordPlus | CALCIUM-BINDING PROTEINS | - |
dc.subject.keywordPlus | INTESTINAL INFLAMMATION | - |
dc.subject.keywordPlus | MULTIPLE-SCLEROSIS | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.subject.keywordPlus | CROHNS-DISEASE | - |
dc.subject.keywordPlus | SERUM-LEVELS | - |
dc.subject.keywordPlus | IL-6 | - |
dc.subject.keywordPlus | MRP14 | - |
dc.subject.keywordPlus | NEUTROPHILS | - |
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