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Large-scale production of membrane proteins in saccharomyces cerevisiae: Using a green fluorescent protein fusion strategy in the production of membrane proteins
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Cited 4 time in Scopus
- Authors
- Issue Date
- 2012-02
- Publisher
- Humana Press, Inc.
- Citation
- Methods in molecular biology (Clifton, N.J.), Vol.866, pp.209-216
- Abstract
- The production of membrane proteins in the large quantities necessary for structural analysis requires many optimization steps. The GFP-fusion-based scheme described in earlier chapters ( Chapters 4 , 8 , and 16 ) facilitates these steps by allowing the selection of high yielding clones that produce detergent-stable membrane proteins. Here, we describe the experimental steps required to establish the reproducible, large-scale production and purification of membrane protein-GFP fusions using S. cerevisiae. © 2012 Springer Science+business Media, LLC.
- ISSN
- 1064-3745
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