Publications
Detailed Information
Differentiation of skin sensitizers from irritant chemicals by interleukin-1α and macrophage inflammatory protein-2 in murine keratinocytes : Differentiation of skin sensitizers from irritant chemicals by interleukin-1 alpha and macrophage inflammatory protein-2 in murine keratinocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Son, Dain | - |
dc.contributor.author | Na, Yirang | - |
dc.contributor.author | Cho, Wan-Seob | - |
dc.contributor.author | Lee, Byoung-Hee | - |
dc.contributor.author | Heo, Yong | - |
dc.contributor.author | Park, Jae-Hak | - |
dc.contributor.author | Seok, Seung Hyeok | - |
dc.date.accessioned | 2023-07-07T08:00:39Z | - |
dc.date.available | 2023-07-07T08:00:39Z | - |
dc.date.created | 2020-07-22 | - |
dc.date.created | 2020-07-22 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.citation | Toxicology Letters, Vol.216 No.1, pp.65-71 | - |
dc.identifier.issn | 0378-4274 | - |
dc.identifier.uri | https://hdl.handle.net/10371/194801 | - |
dc.description.abstract | The development of novel alternative testing methods is required to identify the sensitizing capacity of chemicals as a replacement for animal experimentation. We aimed to evaluate in vitro assays as screening tools for detecting skin sensitizers. The murine epidermal keratinocyte cell line HEL-30 was exposed to 16 relevant skin sensitizers and 6 skin irritants. The dose causing 75% cell viability (CV75) measured by an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was chosen as a highest dose and three more doses (0.5 x, 0.1 x, and 0.01 x of CV75) were tested. As parameters, levels of interleukin 1 alpha (IL-1 alpha), macrophage inflammatory protein 2 (MIP-2), IL-6, and IL-18 production were measured using 4 different doses. The accuracy of detecting sensitizers or irritants by IL-1 alpha or MIP-2 alone was exactly same: 75% (12 out of 16) for sensitizers, 83% (5 out of 6) for irritants, and overall 77% (17 out of 22). However, combination of IL-1 alpha and MIP-2 showed better accuracy: 94% (15 out of 16), 67% (4 out of 6), and overall 86% (19 out of 22). IL-6 and IL-18 could not differentiate sensitizers from irritants. This study suggests that the combination of pro-inflammatory cytokines IL-1 alpha and MIP-2 in murine HEL-30 cells can be a reliable in vitro method for identifying chemicals that may act as skin sensitizers. (c) 2012 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Differentiation of skin sensitizers from irritant chemicals by interleukin-1α and macrophage inflammatory protein-2 in murine keratinocytes | - |
dc.title.alternative | Differentiation of skin sensitizers from irritant chemicals by interleukin-1 alpha and macrophage inflammatory protein-2 in murine keratinocytes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.toxlet.2012.10.017 | - |
dc.citation.journaltitle | Toxicology Letters | - |
dc.identifier.wosid | 000312532400009 | - |
dc.identifier.scopusid | 2-s2.0-84870913188 | - |
dc.citation.endpage | 71 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 65 | - |
dc.citation.volume | 216 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Jae-Hak | - |
dc.contributor.affiliatedAuthor | Seok, Seung Hyeok | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | LYMPH-NODE ASSAY | - |
dc.subject.keywordPlus | HUMAN MAXIMIZATION TEST | - |
dc.subject.keywordPlus | EAR SWELLING TEST | - |
dc.subject.keywordPlus | 4-H PATCH TEST | - |
dc.subject.keywordPlus | CONTACT ALLERGENS | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | POTENCY | - |
dc.subject.keywordAuthor | HEL-30 | - |
dc.subject.keywordAuthor | Interleukin-1 alpha | - |
dc.subject.keywordAuthor | Irritant Macrophage inflammatory protein-2 | - |
dc.subject.keywordAuthor | Murine keratinocyte | - |
dc.subject.keywordAuthor | Skin sensitizer | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.