Publications
Detailed Information
A new 3D reconstituted human corneal epithelium model as an alternative method for the eye irritation test
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Kyoung-Mi | - |
dc.contributor.author | Lee, Su-Hyon | - |
dc.contributor.author | Ryu, Yang-Hwan | - |
dc.contributor.author | Jang, Won-Hee | - |
dc.contributor.author | Jung, Haeng-Sun | - |
dc.contributor.author | Han, Ju-Hee | - |
dc.contributor.author | Seok, Seung-Hyeok | - |
dc.contributor.author | Park, Jae-Hak | - |
dc.contributor.author | Son, Youngsook | - |
dc.contributor.author | Park, Young-Ho | - |
dc.contributor.author | Lim, Kyung-Min | - |
dc.date.accessioned | 2023-07-07T08:01:11Z | - |
dc.date.available | 2023-07-07T08:01:11Z | - |
dc.date.created | 2020-08-14 | - |
dc.date.issued | 2011-02 | - |
dc.identifier.citation | Toxicology in Vitro, Vol.25 No.1, pp.403-410 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | https://hdl.handle.net/10371/194813 | - |
dc.description.abstract | Many efforts are being made to develop new alternative in vitro test methods for the eye irritation test. Here we report a new reconstructed human corneal epithelial model (MCTT HCE model) prepared from primary-cultured human limbal epithelial cells as a new alternative in vitro eye irritation test method. In histological and immunohistochemical observation, MCTT HCE model displayed a morphology and bio-marker expressions similar to intact human cornea. Moreover, the barrier function was well preserved as measured by high transepithelial electrical resistance, effective time-50 for Triton X-100, and corneal thickness. To employ the model as a new alternative method for eye irritation test, protocol refinement was performed and optimum assay condition was determined including treatment time, treatment volume, post-incubation time and rinsing method. Using the refined protocol, 25 reference chemicals with known eye irritation potentials were tested. With the viability cut-off value at 50%, chemicals were classified to irritant or non-irritant. When compared with GHS classification, the MCTT HCE model showed the accuracy of 88%, sensitivity of 100% and specificity of 77%. These results suggest that the MCTT HCE model might be useful as a new alternative eye irritation test method. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | A new 3D reconstituted human corneal epithelium model as an alternative method for the eye irritation test | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.tiv.2010.10.019 | - |
dc.citation.journaltitle | Toxicology in Vitro | - |
dc.identifier.wosid | 000287010600048 | - |
dc.identifier.scopusid | 2-s2.0-78650521308 | - |
dc.citation.endpage | 410 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 403 | - |
dc.citation.volume | 25 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Seok, Seung-Hyeok | - |
dc.contributor.affiliatedAuthor | Park, Jae-Hak | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | OCULAR SURFACE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | OPACITY | - |
dc.subject.keywordAuthor | MCTT HCE model | - |
dc.subject.keywordAuthor | Eye irritation test | - |
dc.subject.keywordAuthor | Barrier function | - |
dc.subject.keywordAuthor | Cell viability assay | - |
dc.subject.keywordAuthor | Protocol refinement | - |
dc.subject.keywordAuthor | Predictive model | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.