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Highly expressed recombinant human follicle-stimulating hormone from Chinese hamster ovary cells grown in serum-free medium and its effect on induction of folliculogenesis and ovulation

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dc.contributor.authorKim, Dong-Jae-
dc.contributor.authorSeok, Seung-Hyeok-
dc.contributor.authorBaek, Min-Won-
dc.contributor.authorLee, Hui-Young-
dc.contributor.authorJuhn, Jae-Hyeon-
dc.contributor.authorLee, Seungwon-
dc.contributor.authorYun, Minae-
dc.contributor.authorPark, Jae-Hak-
dc.date.accessioned2023-07-07T08:01:30Z-
dc.date.available2023-07-07T08:01:30Z-
dc.date.created2022-04-07-
dc.date.created2022-04-07-
dc.date.issued2010-05-
dc.identifier.citationFertility and Sterility, Vol.93 No.8, pp.2652-2660-
dc.identifier.issn0015-0282-
dc.identifier.urihttps://hdl.handle.net/10371/194820-
dc.description.abstractObjective: To develop efficient Chinese hamster ovary (CHO) cells that express recombinant human FSH (rhFSH) in serum-free conditions and to investigate the effect of this newly synthesized rhFSH on folliculogenesis and ovulation. Design: Experimental study. Setting: Seoul National University, South Korea. Animals: Forty immature hypophysectomized rats and 40 androgen-sterilized mice. Intervention(s): A stable single CHO cell that expresses rhFSH at a high level was obtained by introducing the human chorionic gonadotropin (hCG) alpha-subunit and FSH beta-subunit genes. After purification processing, we investigated the effect of this newly synthesized rhFSH on folliculogenesis in hypophysectomized rats and ovulation in androgen-sterilized mice. Main Outcome Measure(s): The ovary weight, uterine weight, number of follicles, and ovarian morphology were evaluated in immature hypophysectomized rats. The number of ovulated oocytes and ovarian morphology were examined in androgen-sterilized mice. Result(s): After purification processing, we analyzed the new rhFSH using matrix-associated laser desorption ionization-time of flight and found that this new rhFSH increased both ovarian weight and uterine weight in hypophysectomized rats and induced ovulation in androgen-sterilized mice. Conclusion(s): This newly synthesized rhFSH might be safely used in anovulatory infertile woman as well as in ovulation induction protocols for subfertile women. (Fertil Steril (R) 2010; 93: 2652-60. (C) 2010 by American Society for Reproductive Medicine.)-
dc.language영어-
dc.publisherElsevier BV-
dc.titleHighly expressed recombinant human follicle-stimulating hormone from Chinese hamster ovary cells grown in serum-free medium and its effect on induction of folliculogenesis and ovulation-
dc.typeArticle-
dc.identifier.doi10.1016/j.fertnstert.2009.05.009-
dc.citation.journaltitleFertility and Sterility-
dc.identifier.wosid000277608700027-
dc.identifier.scopusid2-s2.0-77952321019-
dc.citation.endpage2660-
dc.citation.number8-
dc.citation.startpage2652-
dc.citation.volume93-
dc.description.isOpenAccessY-
dc.contributor.affiliatedAuthorSeok, Seung-Hyeok-
dc.contributor.affiliatedAuthorPark, Jae-Hak-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusIN-VITRO FERTILIZATION-
dc.subject.keywordPlusPURIFIED URINARY FSH-
dc.subject.keywordPlusMAMMALIAN-CELLS-
dc.subject.keywordPlusFEMALE RATS-
dc.subject.keywordPlusLH-
dc.subject.keywordPlusSTEROIDOGENESIS-
dc.subject.keywordPlusMULTICENTER-
dc.subject.keywordPlusGONAL-F(R)-
dc.subject.keywordPlusMATURATION-
dc.subject.keywordPlusPREGNANCY-
dc.subject.keywordAuthorRecombinant human FSH-
dc.subject.keywordAuthorfolliculogenesis-
dc.subject.keywordAuthorovulation-
dc.subject.keywordAuthorhypophysectomized rats-
dc.subject.keywordAuthorandrogen-sterilized mice-
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  • College of Veterinary Medicine
  • Department of Veterinary Medicine
Research Area Laboratory Animal Medicine, Toxicologic Pathology

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