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Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae
DC Field | Value | Language |
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dc.contributor.author | Park, Sungwoo | - |
dc.contributor.author | Cho, Sung Won | - |
dc.contributor.author | Lee, Yungyu | - |
dc.contributor.author | Choi, Mincheol | - |
dc.contributor.author | Yang, Jina | - |
dc.contributor.author | Lee, Hojun | - |
dc.contributor.author | Seo, Sang Woo | - |
dc.date.accessioned | 2023-12-11T00:52:37Z | - |
dc.date.available | 2023-12-11T00:52:37Z | - |
dc.date.created | 2021-09-06 | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Computational and Structural Biotechnology Journal, Vol.19, pp.1531-1540 | - |
dc.identifier.issn | 2001-0370 | - |
dc.identifier.uri | https://hdl.handle.net/10371/197858 | - |
dc.description.abstract | Macroalgae is regarded as a promising third-generation marine biomass that can be utilized as a sustainable feedstock for bio-industry due to the high sugar level and absence of lignin. Alginate, composed of 1,4-linked D-mannuronate (M) and L-guluronate (G), is one of the major carbohydrates in brown macroalgae. It is difficult to be assimilated by most industrial microorganisms. Therefore, developing engineered microorganisms that can utilize alginate as a feedstock in order to produce natural products from marine biomass is critical. In this study, we isolated, characterized, and sequenced Vibrio sp. SP1 which rapidly grows using alginate as a sole carbon source. We further engineered this strain by introducing genes encoding enzymes under the control of synthetic expression cassettes to produce lycopene and beta-carotene which are attractive phytochemicals owing to the antioxidant property. We confirmed that the engineered Vibrio sp. SP1 could successfully produce 2.13 +/- 0.37 mg L-1 of lycopene, 2.98 +/- 0. 43 mg L-1 of beta-carotene, respectively, from 10 g L-1 of alginate as a sole carbon source. Furthermore, our engineered strain could directly convert 60 g L-1 of brown macroalgae Sargassum fusiforme into 1.23 +/- 0.21 mg L-1 of lycopene without any pretreatment which had been vitally required for the previous productions. As the first demonstrated strain to produce high-value product from Sargassum, Vibrio sp. SP1 is evaluated to be a desirable platform for the brown macroalgae-based biorefinery. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. | - |
dc.language | 영어 | - |
dc.publisher | Research Network of Computational and Structural Biotechnology | - |
dc.title | Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.csbj.2021.03.007 | - |
dc.citation.journaltitle | Computational and Structural Biotechnology Journal | - |
dc.identifier.wosid | 000684869500002 | - |
dc.identifier.scopusid | 2-s2.0-85102889364 | - |
dc.citation.endpage | 1540 | - |
dc.citation.startpage | 1531 | - |
dc.citation.volume | 19 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Seo, Sang Woo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | LYCOPENE PRODUCTION | - |
dc.subject.keywordPlus | ISOPENTENYL DIPHOSPHATE | - |
dc.subject.keywordPlus | BIOFUEL PRODUCTION | - |
dc.subject.keywordPlus | GENERATION-TIME | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PRETREATMENT | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | NATRIEGENS | - |
dc.subject.keywordAuthor | Macroalgae | - |
dc.subject.keywordAuthor | Biorefinery | - |
dc.subject.keywordAuthor | Alginate | - |
dc.subject.keywordAuthor | Vibrio sp. SP1 | - |
dc.subject.keywordAuthor | Carotenoids | - |
dc.subject.keywordAuthor | Genome sequencing | - |
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