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Effects of ascorbic acid on alpha-L-arabinofuranosidase and alpha-L-arabinopyranosidase activities from Bifidobacterium longum RD47 and its application to whole cell bioconversion of ginsenoside

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Ku, Seockmo; You, Hyun Ju; Park, Myeong Soo; Ji, Geun Eog

Issue Date
The Korean Society for Applied Biological Chemistry
Journal of the Korean Society for Applied Biological Chemistry, Vol.58 No.6, pp.857-865
Bifidobacterium longum RD47 was cultured in 24 kinds of modified MRS broths containing various ingredients to select the most promising source that induces microbial enzymes. Among the various ingredients, ascorbic acid significantly enhanced alpha-l-arabinofuranosidase and alpha-l-arabinopyranosidase activities in Bifidobacterium longum RD47. Addition of 2 % ascorbic acid (w/v) to MRS showed the maximum enzyme activities. Both whole cell and disrupted cell homogenates showed efficient rho-nitrophenyl-beta-d-glucopyranoside and rho-nitrophenyl-beta-d-glucofuranoside hydrolysis activities. The initially enhanced alpha-l-arabinopyranosidase and alpha-l-arabinofuranosidase activities by ascorbic acid were maintained over the cell disruption process. The optimal pH of alpha-l-arabinofuranosidase and alpha-l-arabinopyranosidase was 5.0 and 7.0, respectively. Both enzymes showed the maximum activities at 40.0 A degrees C. Under the controlled condition using Bifidobacterium longum RD47, ginsenoside Rb2, and Rc were converted to ginsenoside Rd.
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  • College of Human Ecology
  • Department of Food and Nutrition
Research Area Biochemistry & Molecular Biology, Food Science & Technology, Microbiology, 미생물학, 분자생물학, 식품공학


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