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Techniques to Study Automethylation of Histone Methyltransferases and its Functional Impact
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- Authors
- Issue Date
- 2022-01
- Publisher
- Humana Press, Inc.
- Citation
- Methods in molecular biology (Clifton, N.J.), Vol.2529, pp.109-120
- Abstract
- The catalytic activity of histone methyltransferases is not restricted to histones but also includes noncanonical substrates. Increasing evidence shows that histone methyltransferases methylate themselves, and automethylation has emerged as a self-regulatory mechanism. Here, we introduce experimental procedures to identify automethylation sites of histone methyltransferases and to investigate the function of automethylation in a reconstituted biochemical system and in cellular contexts.
- ISSN
- 1064-3745
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