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C-terminal de novo sequencing of peptides using oxazolone-based derivatization with bromine signature
Cited 16 time in
Web of Science
Cited 17 time in Scopus
- Authors
- Issue Date
- 2011-12
- Publisher
- Academic Press
- Citation
- Analytical Biochemistry, Vol.419 No.2, pp.211-216
- Abstract
- Due to almost identical chemical properties of C-terminal and side-chain carboxylic groups, selective C-terminal derivatization has been difficult. Although oxazolone-based C-terminal derivatization is the only selective C-terminal modification available, it has not been used widely because of its low derivatization efficiency. In this paper, an improved oxazolone chemistry for incorporation of Br signature to C-terminus is reported. MS/MS analysis of the brominated peptides led to a series of y ions with Br signature, facilitating de novo C-terminal sequencing. (C) 2011 Elsevier Inc. All rights reserved.
- ISSN
- 0003-2697
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