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Down-regulation of IL-7Rα expression in human T cells via DNA methylation
DC Field | Value | Language |
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dc.contributor.author | Kim, Hang-Rae | - |
dc.contributor.author | Hwang, Kyung-A | - |
dc.contributor.author | Kim, Ki-Chan | - |
dc.contributor.author | Kang, Insoo | - |
dc.date.accessioned | 2024-05-16T01:33:59Z | - |
dc.date.available | 2024-05-16T01:33:59Z | - |
dc.date.created | 2024-04-18 | - |
dc.date.created | 2024-04-18 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.citation | Journal of Immunology, Vol.178 No.9, pp.5473-5479 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://hdl.handle.net/10371/202698 | - |
dc.description.abstract | IL-7 is critical for the development and survival of T cells. Recently, we found two subsets of human CD8(+) T cells expressing IL-7R alpha(high) and IL-7R alpha(low) with different cell survival responses to IL-7. Although these CD8(+) T cell subsets have differential IL-7R alpha gene expression, the mechanism for this is unknown. DNA methylation is an important gene regulatory mechanism and is associated with the inactivation of gene expression. Thus, we investigated a role for DNA methylation in differentially regulating IL-7R alpha gene expression in human CD8(+) T cells and Jurkat T cells. IL-7R alpha(high)CD8+ T cells had decreased methylation in the IL-7R alpha gene promoter compared with IL-7R alpha(low)CD8(+) T cells and Jurkat T cells with low levels of IL-7R alpha. Treating Jurkat T cells with 5-aza-2'-deoxycytidine, which reduced DNA methylation, increased IL-7R alpha expression. Plus, the unmethylated IL-7R alpha gene promoter construct had higher levels of promoter activity than the methylated one as measured by a luciferase reporter assay. These findings suggest that DNA methylation is involved in regulating IL-7R alpha expression in T cells via affecting IL-7R alpha gene promoter activity, and that the methylation of this gene promoter could be a potential target for modifying IL-7-mediated T cell development and survival. | - |
dc.language | 영어 | - |
dc.publisher | American Association of Immunologists | - |
dc.title | Down-regulation of IL-7Rα expression in human T cells via DNA methylation | - |
dc.type | Article | - |
dc.identifier.doi | 10.4049/jimmunol.178.9.5473 | - |
dc.citation.journaltitle | Journal of Immunology | - |
dc.identifier.wosid | 000246054400010 | - |
dc.identifier.scopusid | 2-s2.0-34247639478 | - |
dc.citation.endpage | 5479 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | 5473 | - |
dc.citation.volume | 178 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Hang-Rae | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IFN-GAMMA GENE | - |
dc.subject.keywordPlus | CHROMATIN-STRUCTURE | - |
dc.subject.keywordPlus | MEMORY CELLS | - |
dc.subject.keywordPlus | INTERLEUKIN-7 | - |
dc.subject.keywordPlus | IL-7 | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | HOMEOSTASIS | - |
dc.subject.keywordPlus | NAIVE | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | CANCER | - |
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