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METTL3 regulates alternative splicing of cell cycle-related genes via crosstalk between mRNA m(6)A modifications and splicing factors
DC Field | Value | Language |
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dc.contributor.author | Kim, Yeongjoo | - |
dc.contributor.author | Shin, Seungjae | - |
dc.contributor.author | Kwon, Sunyoung | - |
dc.contributor.author | Moon, Kisung | - |
dc.contributor.author | Baek, Su -Vin | - |
dc.contributor.author | Jo, Ahyoung | - |
dc.contributor.author | Kim, Hyung-Sik | - |
dc.contributor.author | Hwang, Gue-Ho | - |
dc.contributor.author | Bae, Sang Su | - |
dc.contributor.author | Kim, Yun Hak | - |
dc.contributor.author | Cho, Sung Yup | - |
dc.contributor.author | Oh, Jung-Min | - |
dc.date.accessioned | 2024-05-16T01:37:27Z | - |
dc.date.available | 2024-05-16T01:37:27Z | - |
dc.date.created | 2023-06-19 | - |
dc.date.created | 2023-06-19 | - |
dc.date.created | 2023-06-19 | - |
dc.date.issued | 2023-04 | - |
dc.identifier.citation | American Journal of Cancer Research, Vol.13 No.4, pp.1443-1456 | - |
dc.identifier.issn | 2156-6976 | - |
dc.identifier.uri | https://hdl.handle.net/10371/202727 | - |
dc.description.abstract | N-6-methyladenosine (m(6)A) modification in RNA affects various aspects of RNA metabolism and regulates gene expression. This modification is modulated by many regulatory proteins, such as m(6)A methyltransferases (writ-ers), m(6)A demethylases (erasers), and m(6)A-binding proteins (readers). Previous studies have suggested that al-terations in m6A regulatory proteins induce genome-wide alternative splicing in many cancer cells. However, the functional effects and molecular mechanisms of m6A-mediated alternative splicing have not been fully elucidated. To understand the consequences of this modification on RNA splicing in cancer cells, we performed RNA sequenc-ing and analyzed alternative splicing patterns in METTL3-knockdown osteosarcoma U2OS cells. We detected 1,803 alternatively spliced genes in METTL3-knockdown cells compared to the controls and found that cell cycle-related genes were enriched in differentially spliced genes. A comparison of the published MeRIP-seq data for METTL14 with our RNA sequencing data revealed that 70-87% of alternatively spliced genes had an m6A peak near 1 kb of alternative splicing sites. Among the 19 RNA-binding proteins enriched in alternative splicing sites, as revealed by motif analysis, expression of SFPQ highly correlated with METTL3 expression in 12,839 TCGA pan-cancer patients. We also found that cell cycle-related genes were enriched in alternatively spliced genes of other cell lines with METTL3 knockdown. Taken together, we suggest that METTL3 regulates m(6)A-dependent alternative splicing, espe-cially in cell cycle-related genes, by regulating the functions of splicing factors such as SFPQ. | - |
dc.language | 영어 | - |
dc.publisher | e-Century Publishing Corporation | - |
dc.title | METTL3 regulates alternative splicing of cell cycle-related genes via crosstalk between mRNA m(6)A modifications and splicing factors | - |
dc.type | Article | - |
dc.citation.journaltitle | American Journal of Cancer Research | - |
dc.identifier.wosid | 000982232900018 | - |
dc.citation.endpage | 1456 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 1443 | - |
dc.citation.volume | 13 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Bae, Sang Su | - |
dc.contributor.affiliatedAuthor | Cho, Sung Yup | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PLOTTING SERVER | - |
dc.subject.keywordPlus | MAP ANALYSIS | - |
dc.subject.keywordPlus | NUCLEAR-RNA | - |
dc.subject.keywordPlus | N-6-METHYLADENOSINE | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | PSF | - |
dc.subject.keywordAuthor | Alternative splicing | - |
dc.subject.keywordAuthor | cell cycle | - |
dc.subject.keywordAuthor | m6A modification | - |
dc.subject.keywordAuthor | METTL3 | - |
dc.subject.keywordAuthor | splicing factor | - |
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