Publications

Detailed Information

Differential incorporation of biotinylated polyamines by transglutaminase 2

DC Field Value Language
dc.contributor.authorJeon, Ju Hong-
dc.contributor.authorKim, Chai Wan-
dc.contributor.authorShin, Dong Myung-
dc.contributor.authorKim, Kyu il-
dc.contributor.authorCho, Sung Yup-
dc.contributor.authorKwon, Joon Cheo-
dc.contributor.authorChoi, Kyung Ho-
dc.contributor.authorKang, Heun Soo-
dc.contributor.authorKim, In Gyu-
dc.date.accessioned2024-05-16T01:41:08Z-
dc.date.available2024-05-16T01:41:08Z-
dc.date.created2024-04-23-
dc.date.created2024-04-23-
dc.date.created2024-04-23-
dc.date.issued2003-01-
dc.identifier.citationFEBS Letters, Vol.534 No.1-3, pp.180-184-
dc.identifier.issn0014-5793-
dc.identifier.urihttps://hdl.handle.net/10371/202788-
dc.description.abstractPolyamine incorporation or cross-linking of proteins, post-translational modifications mediated by transglutaminase 2 (Tease 2), have been implicated in a variety of physiological functions including cell adhesion, extracellular matrix formation and apoptosis. To better understand the intracellular regulation mechanism of Tease 2, the properties of biotinylated polyamines as substrates for determining in situ Tease activity were analyzed. We synthesized biotinylated spermine (BS), and compared the in vitro and in situ incorporation of BS with that of biotinylated pentylamine (BP), which is an artificial polyamine derivative. When measured in vitro, BP showed a significantly higher incorporation rate than BS. In contrast, in situ incorporation of both BS and BP was not detected even in Tease 2-overexpressed 293 cells. Cells exposed to high calcium showed a marked increase of BP incorporation but not of BS. These data indicate that the in situ activity of Tease 2 gives different results with different substrates, and suggest the possibility of overrepresentation of in situ Tease 2 activity when assayed with BP. Therefore, careful interpretation or evaluation of in situ Tease 2 activity may be required. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.-
dc.language영어-
dc.publisherElsevier BV-
dc.titleDifferential incorporation of biotinylated polyamines by transglutaminase 2-
dc.typeArticle-
dc.identifier.doi10.1016/S0014-5793(02)03836-X-
dc.citation.journaltitleFEBS Letters-
dc.identifier.wosid000180502100032-
dc.identifier.scopusid2-s2.0-0037448439-
dc.citation.endpage184-
dc.citation.number1-3-
dc.citation.startpage180-
dc.citation.volume534-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorJeon, Ju Hong-
dc.contributor.affiliatedAuthorCho, Sung Yup-
dc.contributor.affiliatedAuthorKim, In Gyu-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusTISSUE TRANSGLUTAMINASE-
dc.subject.keywordPlusIN-SITU-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusCALCIUM-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusGTP-
dc.subject.keywordPlusMODULATION-
dc.subject.keywordPlusNUCLEAR-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordAuthortransglutaminase 2-
dc.subject.keywordAuthorbiotinylated spermine-
dc.subject.keywordAuthorpolyamine conjugation-
dc.subject.keywordAuthorpost-translational modification-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Related Researcher

  • College of Medicine
Research Area Cancer genomics, Drug resistance, Targeted therapeutics

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share