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GTP is required to stabilize and display transamidation activity of transglutaminase 2
DC Field | Value | Language |
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dc.contributor.author | Jeon, Ju Hong | - |
dc.contributor.author | Cho, Sung Yup | - |
dc.contributor.author | Kim, Chai Wan | - |
dc.contributor.author | Shin, Dong Mi | - |
dc.contributor.author | Kweon, Joon Chul | - |
dc.contributor.author | Choi, Kyung Ho | - |
dc.contributor.author | Park, Sang Chul | - |
dc.contributor.author | Kim, In Gyu | - |
dc.date.accessioned | 2024-05-16T01:41:12Z | - |
dc.date.available | 2024-05-16T01:41:12Z | - |
dc.date.created | 2024-04-23 | - |
dc.date.created | 2024-04-23 | - |
dc.date.created | 2024-04-23 | - |
dc.date.issued | 2002-06 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, Vol.294 No.4, pp.818-822 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://hdl.handle.net/10371/202789 | - |
dc.description.abstract | Transglutaminase 2 (TGase 2) is a bifunctional enzyme that catalyzes calcium-dependent transamidation and GTP binding/hydrolysis. The transamidation activity is proposed to be associated with several neurodegenerative disorders such as Alzheimer's and Hungtinton's disease. However, the regulation mechanism by which TGase 2 causes neurodegeneration is unknown. In this study, we show that two activities of TGase 2 have a differential stability; transamidation activity is less stable than GTP hydrolytic activity, and that GTP was required to stabilize and to display transamidation activity. Moreover, GTP binding-defective mutant of TGase 2 did not show any transamidation activity in transfection experiments. These results indicate that GTP binding is crucial for transamidation activity of TGase 2, suggesting that protein cross-linking by TGase 2 might be associated with G-protein coupled receptor signaling system. Thus, our data could contribute to understand the regulation of TGase 2 activity and TGase 2-associated pathogenesis. (C) 2002 Elsevier Science (USA). All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | GTP is required to stabilize and display transamidation activity of transglutaminase 2 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0006-291X(02)00582-X | - |
dc.citation.journaltitle | Biochemical and Biophysical Research Communications | - |
dc.identifier.wosid | 000176490100012 | - |
dc.identifier.scopusid | 2-s2.0-0036294738 | - |
dc.citation.endpage | 822 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 818 | - |
dc.citation.volume | 294 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Jeon, Ju Hong | - |
dc.contributor.affiliatedAuthor | Cho, Sung Yup | - |
dc.contributor.affiliatedAuthor | Park, Sang Chul | - |
dc.contributor.affiliatedAuthor | Kim, In Gyu | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HUMAN TISSUE TRANSGLUTAMINASE | - |
dc.subject.keywordPlus | BINDING PROTEIN | - |
dc.subject.keywordPlus | G(H) | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordAuthor | transglutaminase 2 | - |
dc.subject.keywordAuthor | transamidation | - |
dc.subject.keywordAuthor | GTP binding/hydrolysis | - |
dc.subject.keywordAuthor | enzyme stability | - |
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