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Copper Oxide Spike Grids for Enhanced Solution Transfer in Cryogenic Electron Microscopy

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Authors

Lee, Dukwon; Lee, Hansol; Lee, Jinwook; Roh, Soung-Hun; Ha, Nam-Chul

Issue Date
2023-09
Publisher
한국분자세포생물학회
Citation
Molecules and Cells, Vol.46 No.9, pp.538-544
Abstract
The formation of uniform vitreous ice is a crucial step in the preparation of samples for cryogenic electron microscopy (cryo-EM). Despite the rapid technological progress in EM, controlling the thickness of vitreous ice on sample grids with reproducibility remains a major obstacle to obtaining high quality data in cryo-EM imaging. The commonly employed classical blotting process faces the problem of excess water that cannot be absorbed by the filter paper, resulting in the formation of thick and heterogeneous ice. In this study, we propose a novel approach that combines the recently developed nanowire self-wicking technique with the classical blotting method to effectively control the thickness and homogeneity of vitrified ice. With simple procedures, we generated a copper oxide spike (COS) grid by inducing COSs on commercially available copper grids, which can effectively remove excess water during the blotting procedure without damaging the holey carbon membrane. The ice thickness could be controlled with good reproducibility compared to non-oxidized grids. Incorporated into other EM techniques, our new modification method is an effective option for obtaining high-quality data during cryo-EM imaging.
ISSN
1016-8478
URI
https://hdl.handle.net/10371/202892
DOI
https://doi.org/10.14348/molcells.2023.0058
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  • College of Natural Sciences
  • School of Biological Sciences
Research Area Cryogenic Electron Microscopy (Cryo-EM), Structural Biology, 분자생물학, 생물물리학, 생화학

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