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The Effect of Clostridium butyricum on Gut Microbial Changes and Functional Profiles of Metabolism in High-fat Diet–fed Rats Depending on Age and Sex : The Effect of<i> Clostridium</i><i> butyricum</i> on Gut Microbial Changes and Functional Profiles of Metabolism in High-fat Diet-fed Rats Depending on Age and Sex

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Authors

Choi, Soo In; Kim, Nayoung; Choi, Yonghoon; Nam, Ryoung Hee; Jang, Jae Young; Cho, Sung Yup

Issue Date
2024-04
Publisher
대한소화기 기능성질환∙운동학회
Citation
Journal of Neurogastroenterology and Motility (JNM), Vol.30 No.2, pp.236-250
Abstract
Background/Aims A high -fat diet (HFD) causes dysbiosis and promotes inflammatory responses in the colon. This study aims to evaluate the effects of Clostridium butyricum on HFD-induced gut microbial changes in rats. Methods Six -week-old Fischer -344 rats with both sexes were given a control or HFD during 8 weeks, and 1 -to -100 -fold diluted Clostridium butyricum were administered by gavage. Fecal microbiota analyses were conducted using 16S ribosomal RNA metagenomic sequencing and predictive functional profiling of microbial communities in metabolism. Results A significant increase in Ruminococcaceae and Lachnospiraceae, which are butyric acid -producing bacterial families, was observed in the probiotics groups depending on sex. In contrast, Akkermansia muciniphila, which increased through a HFD regardless of sex, and decreased in the probiotics groups. A. muciniphila positively correlated with Claudin-1 expression in males (P < 0.001) and negatively correlated with the expression of Claudin-2 (P = 0.042), IL-1 beta (P = 0.037), and IL -6 (P = 0.044) in females. In terms of functional analyses, a HFD decreased the relative abundances of M00131 (carbohydrate metabolism module), M00579, and M00608 (energy metabolism), and increased those of M00307 (carbohydrate metabolism), regardless of sex. However, these changes recovered especially in male C. butyricum groups. Furthermore, M00131, M00579, and M00608 showed a positive correlation and M00307 showed a negative correlation with the relative abundance of A. muciniphila (P < 0.001). Conclusion The beneficial effects of C. butyricum on HFD-induced gut dysbiosis in young male rats originate from the functional profiles of carbohydrate and energy metabolism.
ISSN
2093-0879
URI
https://hdl.handle.net/10371/203511
DOI
https://doi.org/10.5056/jnm23096
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Research Area Cancer genomics, Drug resistance, Targeted therapeutics

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