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Doxorubicin Induces the Persistent Activation of Intracellular Transglutaminase 2 That Protects from Cell Death

Cited 22 time in Web of Science Cited 22 time in Scopus
Authors

Cho, Sung Yup; Jeong, Eui Man; Lee, Jin-Haeng; Kim, Hyo-Jun; Lim, Jisun; Kim, Chai-Wan; Shin, Dong-Myung; Jeon, Ju-Hong; Choi, Kyungho; Kim, In-Gyu

Issue Date
2012-03
Publisher
한국분자세포생물학회
Citation
Molecules and Cells, Vol.33 No.3, pp.235-241
Abstract
The activation of transglutaminase 2 (TG2), an enzyme that catalyzes post-translational modifications of proteins, has been implicated in apoptosis, cell adhesion and inflammatory responses. We previously reported that intracellular TG2 is activated under oxidative stress conditions, such as ultraviolet irradiation, ischemia-reperfusion, and hypoxia. In this study, we examined the effect of genotoxic stress on the intracellular activity of TG2 using doxorubicin which generates reactive oxygen species that lead to double-strand breakage of DNA. We demonstrated that doxorubicin elicits the persistent activation of TG2. Doxorubicin-induced TG2 activity was suppressed by treatment with caffeine at the early phase, N-acetylcysteine at the mid-phase, and EGTA at the late phase. However, treatment with a blocking antibody against TGF beta or toll-like receptor 2 showed no effect on TG2 activity, indicating that at least three different signaling pathways may be involved in the process of TG2 activation. In addition, using MEF cells defective for TG2 and cells overexpressing an active-site mutant of TG2, we revealed that doxorubicin-induced cell death is inversely correlated with TG2 activity. Our findings indicate that the persistent activation of TG2 by doxorubicin contributes to cell survival, suggesting that the mechanism-based inhibition of TG2 may be a novel strategy to prevent drug-resistance in doxorubicin treatment.
ISSN
1016-8478
URI
https://hdl.handle.net/10371/203540
DOI
https://doi.org/10.1007/s10059-012-2201-9
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  • College of Medicine
Research Area Cancer genomics, Drug resistance, Targeted therapeutics

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