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Transglutaminase 2 inhibits apoptosis induced by calcium-overload through down-regulation of Bax
DC Field | Value | Language |
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dc.contributor.author | Cho, Sung Yup | - |
dc.contributor.author | Lee, Jin-Haeng | - |
dc.contributor.author | Bae, Han-Dong | - |
dc.contributor.author | Jeong, Eui Man | - |
dc.contributor.author | Jang, Gi-Yong | - |
dc.contributor.author | Kim, Chai-Wan | - |
dc.contributor.author | Shin, Dong-Myung | - |
dc.contributor.author | Jeon, Ju-Hong | - |
dc.contributor.author | Kim, In-Gyu | - |
dc.date.accessioned | 2024-05-20T07:30:24Z | - |
dc.date.available | 2024-05-20T07:30:24Z | - |
dc.date.created | 2021-12-21 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.42 No.9, pp.639-650 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://hdl.handle.net/10371/203544 | - |
dc.description.abstract | An abrupt increase of intracellular Ca(2+) is observed in cells under hypoxic or oxidatively stressed conditions. The dysregulated increase of cytosolic Ca(2+) triggers apoptotic cell death through mitochondrial swelling and activation of Ca(2+)-dependent enzymes. Transglutaminase 2 (TG2) is a Ca(2+)-dependent enzyme that catalyzes transamidation reaction producing cross-linked and polyaminated proteins. TG2 activity is known to be involved in the apoptotic process. However, the pro-apoptotic role of TG2 is still controversial. In this study, we investigate the role of TG2 in apoptosis induced by Ca(2+)-overload. Overexpression of TG2 inhibited the A23187-induced apoptosis through suppression of caspase-3 and -9 activities, cytochrome c release into cytosol, and mitochondria membrane depolarization. Conversely, down-regulation of TG2 caused the increases of cell death, caspase-3 activity and cytochrome c in cytosol in response to Ca(2+)-overload. Western blot analysis of Bcl-2 family proteins showed that TG2 reduced the expression level of Bax protein. Moreover, overexpression of Bax abrogated the anti-apoptotic effect of TG2, indicating that TG2-mediated suppression of Bax is responsible for inhibiting cell death under Ca(2+)-overloaded conditions. Our findings revealed a novel anti-apoptotic pathway involving TG2, and suggested the induction of TG2 as a novel strategy for promoting cell survival in diseases such as ischemia and neurodegeneration. | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | - |
dc.title | Transglutaminase 2 inhibits apoptosis induced by calcium-overload through down-regulation of Bax | - |
dc.type | Article | - |
dc.identifier.doi | 10.3858/emm.2010.42.9.063 | - |
dc.citation.journaltitle | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.identifier.wosid | 000282598500005 | - |
dc.identifier.scopusid | 2-s2.0-77957727111 | - |
dc.citation.endpage | 650 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | 639 | - |
dc.citation.volume | 42 | - |
dc.identifier.kciid | ART001480931 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Cho, Sung Yup | - |
dc.contributor.affiliatedAuthor | Jeon, Ju-Hong | - |
dc.contributor.affiliatedAuthor | Kim, In-Gyu | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TISSUE TRANSGLUTAMINASE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | INTRACELLULAR-LOCALIZATION | - |
dc.subject.keywordPlus | MEDIATES ACTIVATION | - |
dc.subject.keywordPlus | MAP KINASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | Bax | - |
dc.subject.keywordAuthor | calcium | - |
dc.subject.keywordAuthor | mitochondria | - |
dc.subject.keywordAuthor | transglutaminase 2 | - |
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