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Improved immunodetection of human papillomavirus E7

Cited 10 time in Web of Science Cited 10 time in Scopus
Authors

Jeon, Ju Hong; Cho, Sung Yup; Kim, CW; Shin, DM; Kwon, JC; Choi, KH; Kim, IG

Issue Date
2002-12
Publisher
Springer Nature
Citation
Experimental & Molecular Medicine, Vol.34 No.6, pp.496-499
Abstract
Human papillomavirus E7 (HPV E7) is a viral oncoprotein that plays an important role in cervical carcinogenesis through binding with retinoblastoma protein (Rb). Inactivation of Rb by E7 is necessary but not sufficient for cellular transformation, suggesting other protein-protein interactions are required for E7-mediated cellular transformation aside from the interaction with Rb. However, studies on the oncogenic function of HPV E7 have been limited by its poor immunoreactivity. In this report, we show that the fixation of purified recombinant HPV E7 on blotted nitrocellulose membrane with glutaldehyde markedly enhanced the immunoreactivity of HPV E7 protein. Using HeLa and Caski cell line which are infected with HPV 18 and HPV 16, respectively, we demonstrated that native HPV E7 proteins also could be detected by this method. These results therefore can provide the experimental conditions for detection of HPV E7 proteins with greater sensitivity and may help to analyze E7 functions.
ISSN
1226-3613
URI
https://hdl.handle.net/10371/203549
DOI
https://doi.org/10.1038/emm.2002.69
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Research Area Cancer genomics, Drug resistance, Targeted therapeutics

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