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In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes

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dc.contributor.authorPark, Subin-
dc.contributor.authorLee, Jangwook-
dc.contributor.authorJo, Mi-hee-
dc.contributor.authorNa, Jin Hee-
dc.contributor.authorPark, Sung-Gurl-
dc.contributor.authorJang, Hyeon-Ki-
dc.contributor.authorKang, Sun-Woong-
dc.contributor.authorKim, Jong-Ho-
dc.contributor.authorKim, Byung-Soo-
dc.contributor.authorPark, Jae Hyung-
dc.contributor.authorKwon, Ick Chan-
dc.contributor.authorRyu, Ju Hee-
dc.contributor.authorKim, Kwangmeyung-
dc.date.accessioned2024-06-13T02:13:17Z-
dc.date.available2024-06-13T02:13:17Z-
dc.date.created2018-06-19-
dc.date.issued2016-07-
dc.identifier.citationAmino Acids, Vol.48 No.7, pp.1641-1654-
dc.identifier.issn0939-4451-
dc.identifier.urihttps://hdl.handle.net/10371/204270-
dc.description.abstractVascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-targeted in vivo imaging approach will be useful for understanding the detailed mechanism of angiogenesis and for evaluating therapeutic angiogenesis.-
dc.language영어-
dc.publisherSpringer Verlag-
dc.titleIn vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes-
dc.typeArticle-
dc.identifier.doi10.1007/s00726-016-2225-0-
dc.citation.journaltitleAmino Acids-
dc.identifier.wosid000377409900010-
dc.identifier.scopusid2-s2.0-84964374593-
dc.citation.endpage1654-
dc.citation.number7-
dc.citation.startpage1641-
dc.citation.volume48-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Byung-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusGLYCOL CHITOSAN NANOPARTICLES-
dc.subject.keywordPlusENDOTHELIAL GROWTH-FACTOR-
dc.subject.keywordPlusMATRIX-METALLOPROTEINASE ACTIVITY-
dc.subject.keywordPlusVEGF RECEPTORS-
dc.subject.keywordPlusCANCER-THERAPY-
dc.subject.keywordPlusDIAGNOSIS-
dc.subject.keywordPlusTISSUE-
dc.subject.keywordPlusVASCULATURE-
dc.subject.keywordPlusDEGRADATION-
dc.subject.keywordPlusDYSFUNCTION-
dc.subject.keywordAuthorVascular endothelial growth factor receptor-
dc.subject.keywordAuthorMatrix metalloproteinase-
dc.subject.keywordAuthorMolecular imaging-
dc.subject.keywordAuthorAngiogenesis-
dc.subject.keywordAuthorFluorescence imaging-
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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