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Non-invasive optical imaging of matrix metalloproteinase activity with albumin-based fluorogenic nanoprobes during angiogenesis in a mouse hindlimb ischemia model

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dc.contributor.authorRyu, Ju Hee-
dc.contributor.authorShin, Jung-Youn-
dc.contributor.authorKim, Sun Ah-
dc.contributor.authorKang, Sun-Woong-
dc.contributor.authorKim, Hyunjoon-
dc.contributor.authorKang, Seokyung-
dc.contributor.authorChoi, Kuiwon-
dc.contributor.authorKwon, Ick Chan-
dc.contributor.authorKim, Byung-Soo-
dc.contributor.authorKim, Kwangmeyung-
dc.date.accessioned2024-06-13T02:15:08Z-
dc.date.available2024-06-13T02:15:08Z-
dc.date.created2018-06-19-
dc.date.created2018-06-19-
dc.date.issued2013-09-
dc.identifier.citationBiomaterials, Vol.34 No.28, pp.6871-6881-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://hdl.handle.net/10371/204304-
dc.description.abstractMatrix metalloproteinase (MMP)-2 and MMP-9 have been known to play the role of essential mediators in angiogenesis. Non-invasive in vivo imaging approach using imaging probes is a potential method of detecting MMP activity in living animals, wherein imaging probes must include the characteristics of non-toxicity, specific targetability, and reasonable signal intensity. Here, we developed MMP-specific and self-quenched human serum albumin (HSA)-based (MMP-HSA) nanoprobes for non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. MMP-specific fluorogenic peptide probes, which were self-quenched with a near-infrared fluorophore and a quencher, were covalently conjugated to HSA (MMP-HSA nanoprobes). MMP-HSA nanoprobes formed stable nanoparticle structures of approximately 36 nm in diameter. Strongly self-quenched MMP-HSA nanoprobes boosted intense fluorescence signals in the presence of MMP-2 and MMP-9. Furthermore, MMP-HSA nanoprobes showed no cytotoxicity in cell culture. Importantly, intravenous injection of MMP HSA nanoprobes provided longer blood half-life and successful non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. In addition, the MMP activity visualized by MMP-HSA nanoprobes was consistent with the results of zymography, Western blot, and immunohistochemistry. MMP-HSA nanoprobes may be useful for monitoring of the initial process of angiogenesis through non-invasive MMP imaging. (C) 2013 Elsevier Ltd. All rights reserved.-
dc.language영어-
dc.publisherPergamon Press Ltd.-
dc.titleNon-invasive optical imaging of matrix metalloproteinase activity with albumin-based fluorogenic nanoprobes during angiogenesis in a mouse hindlimb ischemia model-
dc.typeArticle-
dc.identifier.doi10.1016/j.biomaterials.2013.05.074-
dc.citation.journaltitleBiomaterials-
dc.identifier.wosid000322049200032-
dc.identifier.scopusid2-s2.0-84879461320-
dc.citation.endpage6881-
dc.citation.number28-
dc.citation.startpage6871-
dc.citation.volume34-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Byung-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusHUMAN SERUM-ALBUMIN-
dc.subject.keywordPlusMMP ACTIVITY-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusSURFACE-
dc.subject.keywordPlusCANCER-
dc.subject.keywordPlusPROBE-
dc.subject.keywordPlusMATRIX-METALLOPROTEINASE-9-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusMEMBRANE-
dc.subject.keywordPlusINJURY-
dc.subject.keywordAuthorMatrix metalloproteinase-
dc.subject.keywordAuthorAlbumin-
dc.subject.keywordAuthorMolecular imaging-
dc.subject.keywordAuthorAngiogenesis-
dc.subject.keywordAuthorFluorescence-
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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