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Non-invasive optical imaging of matrix metalloproteinase activity with albumin-based fluorogenic nanoprobes during angiogenesis in a mouse hindlimb ischemia model
DC Field | Value | Language |
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dc.contributor.author | Ryu, Ju Hee | - |
dc.contributor.author | Shin, Jung-Youn | - |
dc.contributor.author | Kim, Sun Ah | - |
dc.contributor.author | Kang, Sun-Woong | - |
dc.contributor.author | Kim, Hyunjoon | - |
dc.contributor.author | Kang, Seokyung | - |
dc.contributor.author | Choi, Kuiwon | - |
dc.contributor.author | Kwon, Ick Chan | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.contributor.author | Kim, Kwangmeyung | - |
dc.date.accessioned | 2024-06-13T02:15:08Z | - |
dc.date.available | 2024-06-13T02:15:08Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2013-09 | - |
dc.identifier.citation | Biomaterials, Vol.34 No.28, pp.6871-6881 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204304 | - |
dc.description.abstract | Matrix metalloproteinase (MMP)-2 and MMP-9 have been known to play the role of essential mediators in angiogenesis. Non-invasive in vivo imaging approach using imaging probes is a potential method of detecting MMP activity in living animals, wherein imaging probes must include the characteristics of non-toxicity, specific targetability, and reasonable signal intensity. Here, we developed MMP-specific and self-quenched human serum albumin (HSA)-based (MMP-HSA) nanoprobes for non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. MMP-specific fluorogenic peptide probes, which were self-quenched with a near-infrared fluorophore and a quencher, were covalently conjugated to HSA (MMP-HSA nanoprobes). MMP-HSA nanoprobes formed stable nanoparticle structures of approximately 36 nm in diameter. Strongly self-quenched MMP-HSA nanoprobes boosted intense fluorescence signals in the presence of MMP-2 and MMP-9. Furthermore, MMP-HSA nanoprobes showed no cytotoxicity in cell culture. Importantly, intravenous injection of MMP HSA nanoprobes provided longer blood half-life and successful non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. In addition, the MMP activity visualized by MMP-HSA nanoprobes was consistent with the results of zymography, Western blot, and immunohistochemistry. MMP-HSA nanoprobes may be useful for monitoring of the initial process of angiogenesis through non-invasive MMP imaging. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Pergamon Press Ltd. | - |
dc.title | Non-invasive optical imaging of matrix metalloproteinase activity with albumin-based fluorogenic nanoprobes during angiogenesis in a mouse hindlimb ischemia model | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.biomaterials.2013.05.074 | - |
dc.citation.journaltitle | Biomaterials | - |
dc.identifier.wosid | 000322049200032 | - |
dc.identifier.scopusid | 2-s2.0-84879461320 | - |
dc.citation.endpage | 6881 | - |
dc.citation.number | 28 | - |
dc.citation.startpage | 6871 | - |
dc.citation.volume | 34 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HUMAN SERUM-ALBUMIN | - |
dc.subject.keywordPlus | MMP ACTIVITY | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE-9 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordAuthor | Matrix metalloproteinase | - |
dc.subject.keywordAuthor | Albumin | - |
dc.subject.keywordAuthor | Molecular imaging | - |
dc.subject.keywordAuthor | Angiogenesis | - |
dc.subject.keywordAuthor | Fluorescence | - |
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