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Modulation of BMP-2-Induced Chondrogenic Versus Osteogenic Differentiation of Human Mesenchymal Stem Cells by Cell-Specific Extracellular Matrices

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dc.contributor.authorKwon, Sun-Hyun-
dc.contributor.authorLee, Tae-Jin-
dc.contributor.authorPark, Jooyeon-
dc.contributor.authorHwang, Ji-Eun-
dc.contributor.authorJin, Min-
dc.contributor.authorJang, Hyeon-Ki-
dc.contributor.authorHwang, Nathaniel S.-
dc.contributor.authorKim, Byung-Soo-
dc.date.accessioned2024-06-13T02:15:20Z-
dc.date.available2024-06-13T02:15:20Z-
dc.date.created2018-06-19-
dc.date.created2018-06-19-
dc.date.issued2013-01-
dc.identifier.citationTissue Engineering - Part A, Vol.19 No.1-2, pp.49-58-
dc.identifier.issn1937-3341-
dc.identifier.urihttps://hdl.handle.net/10371/204308-
dc.description.abstractBone morphogenetic protein-2 (BMP-2) is known to induce both osteogenic and chondrogenic commitment of human mesenchymal stem cells (hMSCs). However, factors influencing BMP-2-dependent chondrogenic and osteogenic differentiation have not been investigated. In this study, we demonstrated that extracellular micro-environments, in the form of cell-derived matrices, play important roles in determining the specific lineage commitment of hMSCs in the presence of BMP-2. Extracellular matrices (ECMs) derived from osteoblasts and chondrocytes were utilized to regulate cell differentiation. Osteogenic and chondrogenic differentiation of hMSCs cultured on the two different cell-derived ECMs were assessed by quantitative real-time-polymerase chain reaction, immunocytochemistry, and western blot analysis. To minimize the effects of the cell-adhesion proteins contained in serum on the ECMs, hMSCs were cultured in serum-free osteogenic or chondrogenic differentiation medium. Fibronectin-, collagen type I-, or collagen type II-coated substrates were utilized as ECM controls. The ECM specific to each cell type promoted lineage-specific commitment of hMSCs in the presence of BMP-2, that is, osteoblast-and chondrocyte-derived ECM promoted osteogenic and chondrogenic commitment, respectively. Therefore, cell-specific ECMs are capable of modulating the BMP-2-induced osteogenic and chondrogenic differentiation of hMSCs.-
dc.language영어-
dc.publisherMary Ann Liebert Inc.-
dc.titleModulation of BMP-2-Induced Chondrogenic Versus Osteogenic Differentiation of Human Mesenchymal Stem Cells by Cell-Specific Extracellular Matrices-
dc.typeArticle-
dc.identifier.doi10.1089/ten.tea.2012.0245-
dc.citation.journaltitleTissue Engineering - Part A-
dc.identifier.wosid000312598400006-
dc.identifier.scopusid2-s2.0-84871264426-
dc.citation.endpage58-
dc.citation.number1-2-
dc.citation.startpage49-
dc.citation.volume19-
dc.description.isOpenAccessY-
dc.contributor.affiliatedAuthorHwang, Nathaniel S.-
dc.contributor.affiliatedAuthorKim, Byung-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusCOLLAGEN-I-
dc.subject.keywordPlusADHESION-
dc.subject.keywordPlusVITRONECTIN-
dc.subject.keywordPlusTRANSPLANTATION-
dc.subject.keywordPlusRHOA-
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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