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Modulation of BMP-2-Induced Chondrogenic Versus Osteogenic Differentiation of Human Mesenchymal Stem Cells by Cell-Specific Extracellular Matrices
DC Field | Value | Language |
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dc.contributor.author | Kwon, Sun-Hyun | - |
dc.contributor.author | Lee, Tae-Jin | - |
dc.contributor.author | Park, Jooyeon | - |
dc.contributor.author | Hwang, Ji-Eun | - |
dc.contributor.author | Jin, Min | - |
dc.contributor.author | Jang, Hyeon-Ki | - |
dc.contributor.author | Hwang, Nathaniel S. | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-13T02:15:20Z | - |
dc.date.available | 2024-06-13T02:15:20Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.citation | Tissue Engineering - Part A, Vol.19 No.1-2, pp.49-58 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204308 | - |
dc.description.abstract | Bone morphogenetic protein-2 (BMP-2) is known to induce both osteogenic and chondrogenic commitment of human mesenchymal stem cells (hMSCs). However, factors influencing BMP-2-dependent chondrogenic and osteogenic differentiation have not been investigated. In this study, we demonstrated that extracellular micro-environments, in the form of cell-derived matrices, play important roles in determining the specific lineage commitment of hMSCs in the presence of BMP-2. Extracellular matrices (ECMs) derived from osteoblasts and chondrocytes were utilized to regulate cell differentiation. Osteogenic and chondrogenic differentiation of hMSCs cultured on the two different cell-derived ECMs were assessed by quantitative real-time-polymerase chain reaction, immunocytochemistry, and western blot analysis. To minimize the effects of the cell-adhesion proteins contained in serum on the ECMs, hMSCs were cultured in serum-free osteogenic or chondrogenic differentiation medium. Fibronectin-, collagen type I-, or collagen type II-coated substrates were utilized as ECM controls. The ECM specific to each cell type promoted lineage-specific commitment of hMSCs in the presence of BMP-2, that is, osteoblast-and chondrocyte-derived ECM promoted osteogenic and chondrogenic commitment, respectively. Therefore, cell-specific ECMs are capable of modulating the BMP-2-induced osteogenic and chondrogenic differentiation of hMSCs. | - |
dc.language | 영어 | - |
dc.publisher | Mary Ann Liebert Inc. | - |
dc.title | Modulation of BMP-2-Induced Chondrogenic Versus Osteogenic Differentiation of Human Mesenchymal Stem Cells by Cell-Specific Extracellular Matrices | - |
dc.type | Article | - |
dc.identifier.doi | 10.1089/ten.tea.2012.0245 | - |
dc.citation.journaltitle | Tissue Engineering - Part A | - |
dc.identifier.wosid | 000312598400006 | - |
dc.identifier.scopusid | 2-s2.0-84871264426 | - |
dc.citation.endpage | 58 | - |
dc.citation.number | 1-2 | - |
dc.citation.startpage | 49 | - |
dc.citation.volume | 19 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Hwang, Nathaniel S. | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | COLLAGEN-I | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | VITRONECTIN | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | RHOA | - |
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