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Therapeutic effects of human adipose stem cell-conditioned medium on stroke

Cited 93 time in Web of Science Cited 100 time in Scopus
Authors

Cho, Yu Jin; Song, Hyeon Suk; Bhang, SukHo; Lee, Saehyoung; Kang, Bong Gu; Lee, Jae Chul; An, Jaeyeol; Cha, Choong Ik; Nam, Do-Hyun; Kim, Byung Soo; Joo, Kyeung Min

Issue Date
2012-09
Publisher
WILEY-BLACKWELL
Citation
JOURNAL OF NEUROSCIENCE RESEARCH, Vol.90 No.9, pp.1794-1802
Abstract
Stem cell therapy is a promising approach for stroke. However, low survival rates and potential tumorigenicity of implanted cells could undermine the efficacy of the cell-based treatment. The use of stem cell-conditioned medium (CM) may be a feasible approach to overcome these limitations. Especially, specific stem cell culture condition and continuous infusion of CM into ischemic brains would have better therapeutic results. The CM was prepared by culturing human adipose-derived stem cells in a three-dimensional spheroid form to increase the secretion of angiogenic/neuroprotective factors. Ischemic stroke was induced by standard middle cerebral artery occlusion methods in the brain of 8-week-old Sprague-Dawley rats. Continuous infusion of CM or aMEM media (0.5 mu l/hr) into the lateral ventricle was initiated 8 days after the surgery and maintained for 7 days. Alteration in the motor function was monitored by the rotarod test. Infarction volume and the number of microvessels or TUNEL-positive neural cells were analyzed 15 days after the surgery. Compared with aMEM, continuous CM infusion reduced the infarction volume and maintained motor function. The number of CD31-positive microvessels and TUNEL-positive neural cells significantly increased and decreased, respectively, in the penumbra regions. Although the apoptosis of all neural cell types decreased, reduction in the microglial apoptosis and astrogliosis was prominent and significant. In this study, the therapeutic effects of the CM against stroke were confirmed in an animal model. Increased endothelial cell proliferation, reduced neural cell apoptosis, and milder astrogliosis may play important roles in the treatment effects of CM. (c) 2012 Wiley Periodicals, Inc.
ISSN
0360-4012
URI
https://hdl.handle.net/10371/204315
DOI
https://doi.org/10.1002/jnr.23063
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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