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3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture
DC Field | Value | Language |
---|---|---|
dc.contributor.author | La, Wan-Geun | - |
dc.contributor.author | Bhang, Suk Ho | - |
dc.contributor.author | Shin, Jung-Youn | - |
dc.contributor.author | Yoon, Hee Hun | - |
dc.contributor.author | Park, Jooyeon | - |
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | Yu, Seung-Ho | - |
dc.contributor.author | Sung, Yung-Eun | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-13T02:16:51Z | - |
dc.date.available | 2024-06-13T02:16:51Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2012-07 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS, Vol.28 No.4, pp.1055-1060 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204320 | - |
dc.description.abstract | In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture. (c) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 28: 10551060, 2012 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | 3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/btpr.1560 | - |
dc.citation.journaltitle | BIOTECHNOLOGY PROGRESS | - |
dc.identifier.wosid | 000307220900020 | - |
dc.identifier.scopusid | 2-s2.0-84864838061 | - |
dc.citation.endpage | 1060 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 1055 | - |
dc.citation.volume | 28 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Sung, Yung-Eun | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | FIBROBLAST ATTACHMENT | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | FIBRONECTIN | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | CHEMISTRY | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | BEHAVIOR | - |
dc.subject.keywordAuthor | cell adhesion | - |
dc.subject.keywordAuthor | cell adhesion molecule | - |
dc.subject.keywordAuthor | dermal fibroblasts | - |
dc.subject.keywordAuthor | 3 | - |
dc.subject.keywordAuthor | 4-dihydroxy-L-phenylalanine | - |
dc.subject.keywordAuthor | serum-free culture | - |
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