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Apatite-Coated Collagen Scaffold for Bone Morphogenetic Protein-2 Delivery
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | La, Wan-Geun | - |
dc.contributor.author | Bhang, Suk Ho | - |
dc.contributor.author | Lee, Tae-Jin | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-13T02:17:52Z | - |
dc.date.available | 2024-06-13T02:17:52Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.citation | TISSUE ENGINEERING PART A, Vol.17 No.17-18, pp.2153-2164 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204339 | - |
dc.description.abstract | Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. BMP-2 is clinically used for spine fusion and bone fracture healing. Commercially available BMP-2 uses a type I collagen scaffold as a carrier, but it only releases BMP-2 for a short period of time, which may release the bone formation efficacy. In the present study, we hypothesize that apatite coating of a collagen scaffold increases the release period as well as the osteogenic efficacy of BMP-2. Apatite coating was achieved by incubating collagen scaffolds in simulated body fluids (SBFs). Apatite coating on collagen scaffolds was confirmed by X-ray diffraction, electron spectroscopy for chemical analysis, attenuated total reflectance-Fourier transform infrared spectroscopy, and scanning electron microscopy. The rate and period of BMP-2 release from apatite-coated collagen scaffolds varied depending on the concentration of SBFs used. The 5x and 10x SBF apatite-coated collagen scaffolds released 91.8%+/- 11.5% and 82.2%+/- 13.1% of their loaded BMP-2 over 13 days in vitro, respectively, whereas noncoated collagen scaffold released 98.3%+/- 2.2% over the initial one day. BMP-2 released from apatite-coated collagen scaffold significantly increased the alkaline phosphatase activity of cultured osteoblasts, compared with BMP-2 released from noncoated collagen scaffold. Computed tomography and histomorphometry showed that BMP-2 delivery using apatite-coated collagen scaffolds resulted in 2.5-fold higher bone formation volume and 4.0-fold higher bone formation area than BMP-2 delivery using noncoated collagen scaffolds. This study shows that simple apatite coating of a collagen scaffold results in a BMP-2 carrier that renders long-term release of BMP-2 and dramatically enhances osteogenic efficacy. | - |
dc.language | 영어 | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | Apatite-Coated Collagen Scaffold for Bone Morphogenetic Protein-2 Delivery | - |
dc.type | Article | - |
dc.identifier.doi | 10.1089/ten.tea.2010.0702 | - |
dc.citation.journaltitle | TISSUE ENGINEERING PART A | - |
dc.identifier.wosid | 000294303700004 | - |
dc.identifier.scopusid | 2-s2.0-80053166656 | - |
dc.citation.endpage | 2164 | - |
dc.citation.number | 17-18 | - |
dc.citation.startpage | 2153 | - |
dc.citation.volume | 17 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CLINICAL-APPLICATIONS | - |
dc.subject.keywordPlus | CONTROLLED-RELEASE | - |
dc.subject.keywordPlus | SUSTAINED-RELEASE | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | EFFICACY | - |
dc.subject.keywordPlus | CARRIERS | - |
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