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Hyaline Cartilage Regeneration by Combined Therapy of Microfracture and Long-Term Bone Morphogenetic Protein-2 Delivery
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | La, Wan-Geun | - |
dc.contributor.author | Bhang, Suk Ho | - |
dc.contributor.author | Kim, Hak-Jun | - |
dc.contributor.author | Im, Gun-Il | - |
dc.contributor.author | Lee, Haeshin | - |
dc.contributor.author | Park, Jung-Ho | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-13T02:17:58Z | - |
dc.date.available | 2024-06-13T02:17:58Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2011-07 | - |
dc.identifier.citation | TISSUE ENGINEERING PART A, Vol.17 No.13-14, pp.1809-1818 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204341 | - |
dc.description.abstract | Microfracture of cartilage induces migration of bone-marrow-derived mesenchymal stem cells. However, this treatment often results in fibrocartilage regeneration. Growth factors such as bone morphogenetic protein (BMP)-2 induce the differentiation of bone-marrow-derived mesenchymal stem cells into chondrocytes, which can be used for hyaline cartilage regeneration. Here, we tested the hypothesis that long-term delivery of BMP-2 to cartilage defects subjected to microfracture results in regeneration of high-quality hyaline-like cartilage, as opposed to short-term delivery of BMP-2 or no BMP-2 delivery. Heparin-conjugated fibrin (HCF) and normal fibrin were used as carriers for the long-and short-term delivery of BMP-2, respectively. Rabbit articular cartilage defects were treated with microfracture combined with one of the following: no treatment, fibrin, short-term delivery of BMP-2, HCF, or long-term delivery of BMP-2. Eight weeks after treatment, histological analysis revealed that the long-term delivery of BMP-2 group (microfracture + HCF + BMP-2) showed the most staining with alcian blue. A biochemical assay, real-time polymerase chain reaction assay and Western blot analysis all revealed that the long-term delivery of BMP-2 group had the highest glucosaminoglycan content as well as the highest expression level of collagen type II. Taken together, the long-term delivery of BMP-2 to cartilage defects subjected to microfracture resulted in regeneration of hyaline-like cartilage, as opposed to short-term delivery or no BMP-2 delivery. Therefore, this method could be more convenient for hyaline cartilage regeneration than autologous chondrocyte implantation due to its less invasive nature and lack of cell implantation. | - |
dc.language | 영어 | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | Hyaline Cartilage Regeneration by Combined Therapy of Microfracture and Long-Term Bone Morphogenetic Protein-2 Delivery | - |
dc.type | Article | - |
dc.identifier.doi | 10.1089/ten.tea.2010.0540 | - |
dc.citation.journaltitle | TISSUE ENGINEERING PART A | - |
dc.identifier.wosid | 000292022300015 | - |
dc.identifier.scopusid | 2-s2.0-79959572220 | - |
dc.citation.endpage | 1818 | - |
dc.citation.number | 13-14 | - |
dc.citation.startpage | 1809 | - |
dc.citation.volume | 17 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | FULL-THICKNESS DEFECTS | - |
dc.subject.keywordPlus | AUTOLOGOUS CHONDROCYTE IMPLANTATION | - |
dc.subject.keywordPlus | STEM-CELL CHONDROGENESIS | - |
dc.subject.keywordPlus | X COLLAGEN GENE | - |
dc.subject.keywordPlus | ARTICULAR-CARTILAGE | - |
dc.subject.keywordPlus | OSTEOCHONDRAL DEFECTS | - |
dc.subject.keywordPlus | CONTROLLED-RELEASE | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | KNEE | - |
dc.subject.keywordPlus | HEPARIN | - |
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