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Combined Gene Therapy with Hypoxia-Inducible Factor-1 alpha and Heme Oxygenase-1 for Therapeutic Angiogenesis : Combined gene therapy with hypoxia-inducible factor-1α and heme oxygenase-1 for therapeutic angiogenesis
DC Field | Value | Language |
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dc.contributor.author | Bhang, Suk Ho | - |
dc.contributor.author | Kim, Ju Hee | - |
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | La, Wan-Geun | - |
dc.contributor.author | Lee, Tae-Jin | - |
dc.contributor.author | Kim, Ga Hee | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-13T02:18:07Z | - |
dc.date.available | 2024-06-13T02:18:07Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2011-04 | - |
dc.identifier.citation | TISSUE ENGINEERING PART A, Vol.17 No.7-8, pp.915-926 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204344 | - |
dc.description.abstract | Transfection with either hypoxia-inducible factor-1 alpha (HIF-1 alpha) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1 alpha gene occurs rapidly, the expressed HIF-1 alpha protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1 alpha and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1 alpha or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1 alpha and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1 alpha gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1 alpha and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy. | - |
dc.language | 영어 | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | Combined Gene Therapy with Hypoxia-Inducible Factor-1 alpha and Heme Oxygenase-1 for Therapeutic Angiogenesis | - |
dc.title.alternative | Combined gene therapy with hypoxia-inducible factor-1α and heme oxygenase-1 for therapeutic angiogenesis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1089/ten.tea.2010.0493 | - |
dc.citation.journaltitle | TISSUE ENGINEERING PART A | - |
dc.identifier.wosid | 000288853300005 | - |
dc.identifier.scopusid | 2-s2.0-79952983093 | - |
dc.citation.endpage | 926 | - |
dc.citation.number | 7-8 | - |
dc.citation.startpage | 915 | - |
dc.citation.volume | 17 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HINDLIMB ISCHEMIA | - |
dc.subject.keywordPlus | PROMOTES ANGIOGENESIS | - |
dc.subject.keywordPlus | CELL TRANSPLANTATION | - |
dc.subject.keywordPlus | LIMB ISCHEMIA | - |
dc.subject.keywordPlus | VEGF | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | NEOVASCULARIZATION | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | VIVO | - |
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