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Combined Gene Therapy with Hypoxia-Inducible Factor-1 alpha and Heme Oxygenase-1 for Therapeutic Angiogenesis : Combined gene therapy with hypoxia-inducible factor-1α and heme oxygenase-1 for therapeutic angiogenesis

DC Field Value Language
dc.contributor.authorBhang, Suk Ho-
dc.contributor.authorKim, Ju Hee-
dc.contributor.authorYang, Hee Seok-
dc.contributor.authorLa, Wan-Geun-
dc.contributor.authorLee, Tae-Jin-
dc.contributor.authorKim, Ga Hee-
dc.contributor.authorKim, Hyun Ah-
dc.contributor.authorLee, Minhyung-
dc.contributor.authorKim, Byung-Soo-
dc.date.accessioned2024-06-13T02:18:07Z-
dc.date.available2024-06-13T02:18:07Z-
dc.date.created2018-06-19-
dc.date.created2018-06-19-
dc.date.issued2011-04-
dc.identifier.citationTISSUE ENGINEERING PART A, Vol.17 No.7-8, pp.915-926-
dc.identifier.issn1937-3341-
dc.identifier.urihttps://hdl.handle.net/10371/204344-
dc.description.abstractTransfection with either hypoxia-inducible factor-1 alpha (HIF-1 alpha) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1 alpha gene occurs rapidly, the expressed HIF-1 alpha protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1 alpha and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1 alpha or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1 alpha and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1 alpha gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1 alpha and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy.-
dc.language영어-
dc.publisherMARY ANN LIEBERT, INC-
dc.titleCombined Gene Therapy with Hypoxia-Inducible Factor-1 alpha and Heme Oxygenase-1 for Therapeutic Angiogenesis-
dc.title.alternativeCombined gene therapy with hypoxia-inducible factor-1α and heme oxygenase-1 for therapeutic angiogenesis-
dc.typeArticle-
dc.identifier.doi10.1089/ten.tea.2010.0493-
dc.citation.journaltitleTISSUE ENGINEERING PART A-
dc.identifier.wosid000288853300005-
dc.identifier.scopusid2-s2.0-79952983093-
dc.citation.endpage926-
dc.citation.number7-8-
dc.citation.startpage915-
dc.citation.volume17-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Byung-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusHINDLIMB ISCHEMIA-
dc.subject.keywordPlusPROMOTES ANGIOGENESIS-
dc.subject.keywordPlusCELL TRANSPLANTATION-
dc.subject.keywordPlusLIMB ISCHEMIA-
dc.subject.keywordPlusVEGF-
dc.subject.keywordPlusDELIVERY-
dc.subject.keywordPlusMODEL-
dc.subject.keywordPlusNEOVASCULARIZATION-
dc.subject.keywordPlusINJURY-
dc.subject.keywordPlusVIVO-
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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