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Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes

DC Field Value Language
dc.contributor.authorLee, Tae-Jin-
dc.contributor.authorBhang, Suk Ho-
dc.contributor.authorLa, Wan-Guen-
dc.contributor.authorYang, Hee Seok-
dc.contributor.authorSeong, Jun Yeup-
dc.contributor.authorLee, Haeshin-
dc.contributor.authorIm, Gun-Il-
dc.contributor.authorLee, Soo-Hong-
dc.contributor.authorKim, Byung-Soo-
dc.date.accessioned2024-06-13T02:18:13Z-
dc.date.available2024-06-13T02:18:13Z-
dc.date.created2018-06-19-
dc.date.created2018-06-19-
dc.date.issued2011-04-
dc.identifier.citationBIOTECHNOLOGY LETTERS, Vol.33 No.4, pp.829-836-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://hdl.handle.net/10371/204346-
dc.description.abstractImplantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting.-
dc.language영어-
dc.publisherSPRINGER-
dc.titleSpinner-flask culture induces redifferentiation of de-differentiated chondrocytes-
dc.typeArticle-
dc.identifier.doi10.1007/s10529-010-0488-1-
dc.citation.journaltitleBIOTECHNOLOGY LETTERS-
dc.identifier.wosid000288548900026-
dc.identifier.scopusid2-s2.0-79952751395-
dc.citation.endpage836-
dc.citation.number4-
dc.citation.startpage829-
dc.citation.volume33-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Byung-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusII COLLAGEN-
dc.subject.keywordPlusMACROPOROUS MICROCARRIERS-
dc.subject.keywordPlusNASAL CHONDROCYTES-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusCARTILAGE-
dc.subject.keywordPlusEXPANSION-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusHYPOXIA-
dc.subject.keywordPlusSYSTEMS-
dc.subject.keywordAuthorChondrocyte-
dc.subject.keywordAuthorDe-differentiation-
dc.subject.keywordAuthorRedifferentiation-
dc.subject.keywordAuthorSpinner-flask culture-
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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