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Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Tae-Jin | - |
dc.contributor.author | Bhang, Suk Ho | - |
dc.contributor.author | La, Wan-Guen | - |
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | Seong, Jun Yeup | - |
dc.contributor.author | Lee, Haeshin | - |
dc.contributor.author | Im, Gun-Il | - |
dc.contributor.author | Lee, Soo-Hong | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-13T02:18:13Z | - |
dc.date.available | 2024-06-13T02:18:13Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2011-04 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, Vol.33 No.4, pp.829-836 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204346 | - |
dc.description.abstract | Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting. | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER | - |
dc.title | Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s10529-010-0488-1 | - |
dc.citation.journaltitle | BIOTECHNOLOGY LETTERS | - |
dc.identifier.wosid | 000288548900026 | - |
dc.identifier.scopusid | 2-s2.0-79952751395 | - |
dc.citation.endpage | 836 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 829 | - |
dc.citation.volume | 33 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | II COLLAGEN | - |
dc.subject.keywordPlus | MACROPOROUS MICROCARRIERS | - |
dc.subject.keywordPlus | NASAL CHONDROCYTES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CARTILAGE | - |
dc.subject.keywordPlus | EXPANSION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordAuthor | Chondrocyte | - |
dc.subject.keywordAuthor | De-differentiation | - |
dc.subject.keywordAuthor | Redifferentiation | - |
dc.subject.keywordAuthor | Spinner-flask culture | - |
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