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Heparin-conjugated fibrin as an injectable system for sustained delivery of bone morphogenetic protein-2

DC Field Value Language
dc.contributor.authorYang, Hee Seok-
dc.contributor.authorLa, Wan-Geun-
dc.contributor.authorBhang, Suk Ho-
dc.contributor.authorJeon, Jeong-Yi-
dc.contributor.authorLee, Jong Ho-
dc.contributor.authorKim, Byung-Soo-
dc.date.accessioned2024-06-13T02:18:48Z-
dc.date.available2024-06-13T02:18:48Z-
dc.date.created2018-06-19-
dc.date.created2018-06-19-
dc.date.issued2010-04-
dc.identifier.citationTissue Engineering - Part A, Vol.16 No.4, pp.1225-1233-
dc.identifier.issn1937-3341-
dc.identifier.urihttps://hdl.handle.net/10371/204357-
dc.description.abstractLong-term release of bone morphogenetic protein-2 (BMP-2) can promote bone regeneration. We developed an injectable system for long-term delivery of BMP-2 by covalently conjugating heparin to fibrinogen. The heparin-conjugated fibrinogen formed an injectable, heparin-conjugated fibrin (HCF) gel when mixed with thrombin. HCF released 89.4 +/- 3.8% of the loaded BMP-2 for 13 days, whereas normal fibrin released 83.7 +/- 7.6% for the initial 3 days. BMP-2 released from HCF significantly increased alkaline phosphatase activity of cultured osteoblasts, whereas BMP-2 released from normal fibrin did not do so, indicating that BMP-2 released from HCF is bioactive and suggesting that long-term delivery of BMP-2 is advantageous over short-term delivery for bone regeneration. HCF, BMP-2-loaded HCF, and BMP-2-loaded normal fibrin containing free heparin were contained in polyester cylindrical tubes and implanted into the hind limb muscle pockets of rats for 8 weeks. Soft X-ray radiography, computed tomography, histomorphometry, calcium assay, and western blot analysis showed that BMP-2-loaded HCF yielded the most extensive bone formation among the groups. Since HCF can deliver BMP-2 over a long term, is an injectable system, and is made of clinically benign materials, this system would have advantages for clinical applications to regenerate bone.-
dc.language영어-
dc.publisherMary Ann Liebert Inc.-
dc.titleHeparin-conjugated fibrin as an injectable system for sustained delivery of bone morphogenetic protein-2-
dc.typeArticle-
dc.identifier.doi10.1089/ten.tea.2009.0390-
dc.citation.journaltitleTissue Engineering - Part A-
dc.identifier.wosid000276356700011-
dc.identifier.scopusid2-s2.0-77950846241-
dc.citation.endpage1233-
dc.citation.number4-
dc.citation.startpage1225-
dc.citation.volume16-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorLee, Jong Ho-
dc.contributor.affiliatedAuthorKim, Byung-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusGROWTH-FACTOR-
dc.subject.keywordPlusCONTROLLED-RELEASE-
dc.subject.keywordPlusOSTEOGENIC EFFICACY-
dc.subject.keywordPlusSPINAL-FUSION-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusREGENERATION-
dc.subject.keywordPlusRHBMP-2-
dc.subject.keywordPlusDEFECTS-
dc.subject.keywordPlusMODEL-
dc.subject.keywordPlusAUGMENTATION-
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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