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Heparin-conjugated fibrin as an injectable system for sustained delivery of bone morphogenetic protein-2
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | La, Wan-Geun | - |
dc.contributor.author | Bhang, Suk Ho | - |
dc.contributor.author | Jeon, Jeong-Yi | - |
dc.contributor.author | Lee, Jong Ho | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-13T02:18:48Z | - |
dc.date.available | 2024-06-13T02:18:48Z | - |
dc.date.created | 2018-06-19 | - |
dc.date.created | 2018-06-19 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | Tissue Engineering - Part A, Vol.16 No.4, pp.1225-1233 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204357 | - |
dc.description.abstract | Long-term release of bone morphogenetic protein-2 (BMP-2) can promote bone regeneration. We developed an injectable system for long-term delivery of BMP-2 by covalently conjugating heparin to fibrinogen. The heparin-conjugated fibrinogen formed an injectable, heparin-conjugated fibrin (HCF) gel when mixed with thrombin. HCF released 89.4 +/- 3.8% of the loaded BMP-2 for 13 days, whereas normal fibrin released 83.7 +/- 7.6% for the initial 3 days. BMP-2 released from HCF significantly increased alkaline phosphatase activity of cultured osteoblasts, whereas BMP-2 released from normal fibrin did not do so, indicating that BMP-2 released from HCF is bioactive and suggesting that long-term delivery of BMP-2 is advantageous over short-term delivery for bone regeneration. HCF, BMP-2-loaded HCF, and BMP-2-loaded normal fibrin containing free heparin were contained in polyester cylindrical tubes and implanted into the hind limb muscle pockets of rats for 8 weeks. Soft X-ray radiography, computed tomography, histomorphometry, calcium assay, and western blot analysis showed that BMP-2-loaded HCF yielded the most extensive bone formation among the groups. Since HCF can deliver BMP-2 over a long term, is an injectable system, and is made of clinically benign materials, this system would have advantages for clinical applications to regenerate bone. | - |
dc.language | 영어 | - |
dc.publisher | Mary Ann Liebert Inc. | - |
dc.title | Heparin-conjugated fibrin as an injectable system for sustained delivery of bone morphogenetic protein-2 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1089/ten.tea.2009.0390 | - |
dc.citation.journaltitle | Tissue Engineering - Part A | - |
dc.identifier.wosid | 000276356700011 | - |
dc.identifier.scopusid | 2-s2.0-77950846241 | - |
dc.citation.endpage | 1233 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 1225 | - |
dc.citation.volume | 16 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Jong Ho | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | CONTROLLED-RELEASE | - |
dc.subject.keywordPlus | OSTEOGENIC EFFICACY | - |
dc.subject.keywordPlus | SPINAL-FUSION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | RHBMP-2 | - |
dc.subject.keywordPlus | DEFECTS | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | AUGMENTATION | - |
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