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Improvement of postnatal neovascularization by human embryonic stem cell-derived endothelial-like cell transplantation in a mouse model of hindlimb ischemia
DC Field | Value | Language |
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dc.contributor.author | Cho, Seung-Woo | - |
dc.contributor.author | Moon, Sung-Hwan | - |
dc.contributor.author | Lee, Soo-Hong | - |
dc.contributor.author | Kang, Sun-Woong | - |
dc.contributor.author | Kim, Jumi | - |
dc.contributor.author | Lim, Jae Min | - |
dc.contributor.author | Kim, Hyo-Soo | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.contributor.author | Chung, Hyung-Min | - |
dc.date.accessioned | 2024-06-13T02:19:54Z | - |
dc.date.available | 2024-06-13T02:19:54Z | - |
dc.date.created | 2018-06-20 | - |
dc.date.created | 2018-06-20 | - |
dc.date.issued | 2007-11 | - |
dc.identifier.citation | Circulation, Vol.116 No.21, pp.2409-2419 | - |
dc.identifier.issn | 0009-7322 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204378 | - |
dc.description.abstract | Background - We established an efficient preparation method to obtain endothelial-like cells (ECs) from human embryonic stem cells (hESCs) and tested whether these hESC-ECs would show therapeutic potential for treatment of hindlimb ischemia. Methods and Results - ECs differentiated from hESCs were obtained by mechanical isolation and cell sorting for von Willebrand factor. The isolated hESC-ECs maintained endothelial cell-specific characteristics such as endothelial marker expression and capillary formation. One day after surgical induction of hindlimb ischemia in athymic mice, hESC-ECs were injected intramuscularly into ischemic limbs. Four weeks after treatment, hESC-EC treatment significantly increased limb salvage (36%) compared with treatment with medium (0%). In addition, laser Doppler imaging showed that the ratio of blood perfusion (ischemic to normal limb) was increased significantly (P < 0.01) by hESC-EC treatment (0.511 +/- 0.167) compared with medium injection (0.073 +/- 0.061). Capillary and arteriole densities were 658 +/- 190/mm(2) and 30 +/- 11/mm(2) in the hESC-EC group, respectively, whereas those in the medium group were 392 +/- 118/mm(2) and 16 +/- 8/mm(2), respectively (P < 0.01). Reverse-transcription polymerase chain reaction with human-specific primers revealed mRNA expression of human endothelial markers and human angiogenic factors in ischemic mouse tissues. The transplanted hESC-ECs were localized as capillaries near muscle tissues in ischemic regions or incorporated in the vessels between muscle tissues, as confirmed by human nuclear antigen staining with platelet/endothelial cell adhesion molecule or von Willebrand factor. Conclusions - This study demonstrates that hESC-EC transplantation improves blood perfusion and limb salvage by facilitating postnatal neovascularization in a mouse model of hindlimb ischemia. Thus, hESC-ECs might be useful as an alternative cell source for angiogenic therapy. | - |
dc.language | 영어 | - |
dc.publisher | Lippincott Williams & Wilkins Ltd. | - |
dc.title | Improvement of postnatal neovascularization by human embryonic stem cell-derived endothelial-like cell transplantation in a mouse model of hindlimb ischemia | - |
dc.type | Article | - |
dc.identifier.doi | 10.1161/CIRCULATIONAHA.106.687038 | - |
dc.citation.journaltitle | Circulation | - |
dc.identifier.wosid | 000251079300008 | - |
dc.identifier.scopusid | 2-s2.0-36348969952 | - |
dc.citation.endpage | 2419 | - |
dc.citation.number | 21 | - |
dc.citation.startpage | 2409 | - |
dc.citation.volume | 116 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Hyo-Soo | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | THERAPEUTIC ANGIOGENESIS | - |
dc.subject.keywordPlus | CARDIAC-FUNCTION | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MYOCARDIUM | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | HEART | - |
dc.subject.keywordAuthor | angiogenesis | - |
dc.subject.keywordAuthor | stem cell transplantation | - |
dc.subject.keywordAuthor | endothelial cells | - |
dc.subject.keywordAuthor | ischemia | - |
dc.subject.keywordAuthor | embryonic stem cells | - |
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