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In vitro chondrocyte culture using hydrophilized PLLA scaffold in bioreactor system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, H.S. | - |
dc.contributor.author | Park, K. | - |
dc.contributor.author | Kim, B.-S. | - |
dc.contributor.author | Kim, J.-J. | - |
dc.contributor.author | Ahn, K.-D. | - |
dc.contributor.author | Han, D.K. | - |
dc.date.accessioned | 2024-06-13T02:20:48Z | - |
dc.date.available | 2024-06-13T02:20:48Z | - |
dc.date.created | 2018-06-21 | - |
dc.date.created | 2018-06-21 | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Key Engineering Materials, Vol.342-343, pp.253-256 | - |
dc.identifier.issn | 1013-9826 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204395 | - |
dc.description.abstract | Nonporous PLLA film and porous PLLA scaffolds were prepared and then grafted with acrylic acid (AA) using in situ direct plasma treatment to obtain PLLA-g-PAA. Chondrocytes isolated from rabbit knee articular cartilages were cultivated in Dulbecco's modified eagle medium-F12 (DMEM-F12) containing 10% fetal bovine serum (FBS) and 1% antibiotics and passaged twice before cell seeding. Once seeded on either PLLA films or scaffolds, they were placed in a bioreactor system and an intermittent hydrodynamic pressure (IHP) was applied in 3 bars, while turned on for 2 min and off for 28 min during 15-day culture. AA grafting to PLLA surface was confirmed from various surface analyses. From WST-1 assay, chondrocyte proliferation was significantly improved with dynamic IHP for PLLA and PLLA-g-PAA scaffolds as compared to static culture. This study indicates that IHP may have significant influence on chondrocytes behavior in 3D culture environment. | - |
dc.language | 영어 | - |
dc.publisher | Trans Tech Publications Ltd. | - |
dc.title | In vitro chondrocyte culture using hydrophilized PLLA scaffold in bioreactor system | - |
dc.type | Article | - |
dc.citation.journaltitle | Key Engineering Materials | - |
dc.identifier.scopusid | 2-s2.0-34147166241 | - |
dc.citation.endpage | 256 | - |
dc.citation.startpage | 253 | - |
dc.citation.volume | 342-343 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, B.-S. | - |
dc.type.docType | Conference Paper | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordAuthor | Acrylic acid | - |
dc.subject.keywordAuthor | Bioreactor | - |
dc.subject.keywordAuthor | Chondrocyte | - |
dc.subject.keywordAuthor | PLLA | - |
dc.subject.keywordAuthor | Tissue engineering | - |
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