Publications
Detailed Information
Skin regeneration using keratinocytes and dermal fibroblasts cultured on biodegradable microspherical polymer scaffolds
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sang‐Soo Kim | - |
dc.contributor.author | So‐Jung Gwak | - |
dc.contributor.author | Choi, Cha Yong | - |
dc.contributor.author | Byung Soo Kim | - |
dc.date.accessioned | 2024-06-13T02:22:29Z | - |
dc.date.available | 2024-06-13T02:22:29Z | - |
dc.date.created | 2018-06-20 | - |
dc.date.created | 2018-06-20 | - |
dc.date.issued | 2005-11 | - |
dc.identifier.citation | Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol.75B No.2, pp.369-377 | - |
dc.identifier.issn | 1552-4973 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204423 | - |
dc.description.abstract | Bioartificial skin sheet grafts have been utilized to treat large burns and chronic ulcers. However, the trypsinization step to harvest cultured skin grafts from culture dishes damages the cells by breaking the anchoring proteins and lowers their uptake ratio after transplantation. In addition, epidermal sheet grafts require a long fabrication period. To overcome these limitations, we utilized biodegradable poly(lactide-co-glycolide) (PLGA) microspheres as both cell culture matrix and transplantation vehicle of skin cells for skin regeneration irt this study. This method could avoid the trypsinization step and have a relatively short preparation period. Human keratinocytes and dermal fibroblasts cultured on PLGA microspheres in spinner flasks proliferated by 3.0-fold and 9.4-fold, respectively, after 10 days. When both types of cells cultured on PLGA microspheres were reinoculated onto culture dishes, the cells migrated from the PLGA microspheres to the culture dish surface, grew, and formed a confluent cell layer within 5 days, showing: the growth and migration abilities of the cells cultured on PLGA microspheres. Full-thickness skin wounds created on the back of athymic mice were either treated with transplantation of keratinocytes and dermal fibroblasts cultured on microspheres (cell-transplanted group), treated with PLGA microspheres alone (microsphere-implanted group), or covered with dressing materials without treatment (untreated group). Three weeks after the treatments, differentiated epithelium that stained positively for cytokeratin, a marker of epidermis, was observed in the cell-transplanted. group, while the microsphere-implanted group and untreated group showed incomplete reepithelialization. Dermal regeneration with positive staining for vimentin , a marker of dermal fibroblast, was observed in the cell-transplanted group. Regenerated dermis with positive staining for vimentin was partly observed in the microsphere-implanted group and untreated group. These results suggest that transplantation of keratinocytes and dermal fibroblasts cultured on PLGA microspheres could be potentially useful as an alternative to bioartificial skin grafts for the treatment of skin wounds. (c) 2005 Wiley Periodicals, Inc. | - |
dc.language | 영어 | - |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | Skin regeneration using keratinocytes and dermal fibroblasts cultured on biodegradable microspherical polymer scaffolds | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jbm.b.30302 | - |
dc.citation.journaltitle | Journal of Biomedical Materials Research - Part B Applied Biomaterials | - |
dc.identifier.wosid | 000233059600018 | - |
dc.identifier.scopusid | 2-s2.0-27744491198 | - |
dc.citation.endpage | 377 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 369 | - |
dc.citation.volume | 75B | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Choi, Cha Yong | - |
dc.contributor.affiliatedAuthor | Byung Soo Kim | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | Cells | - |
dc.subject.keywordPlus | Growth kinetics | - |
dc.subject.keywordPlus | Proteins | - |
dc.subject.keywordPlus | Skin | - |
dc.subject.keywordPlus | Tissue culture | - |
dc.subject.keywordPlus | Transplantation (surgical) | - |
dc.subject.keywordPlus | Dermal fibroblasts | - |
dc.subject.keywordPlus | Keratinocyte | - |
dc.subject.keywordPlus | Poly(lactide-co-glycolide) microspheres | - |
dc.subject.keywordPlus | Skin regeneration | - |
dc.subject.keywordPlus | Tissue engineering | - |
dc.subject.keywordPlus | Dermatology | - |
dc.subject.keywordPlus | cytokeratin | - |
dc.subject.keywordPlus | microsphere | - |
dc.subject.keywordPlus | polyglactin | - |
dc.subject.keywordPlus | polymer | - |
dc.subject.keywordPlus | trypsin | - |
dc.subject.keywordPlus | animal experiment | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | artificial skin | - |
dc.subject.keywordPlus | biodegradable implant | - |
dc.subject.keywordPlus | cell adhesion | - |
dc.subject.keywordPlus | cell growth | - |
dc.subject.keywordPlus | cell migration | - |
dc.subject.keywordPlus | cell proliferation | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | epithelization | - |
dc.subject.keywordPlus | female | - |
dc.subject.keywordPlus | histology | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | immunohistochemistry | - |
dc.subject.keywordPlus | keratinocyte | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | nude mouse | - |
dc.subject.keywordPlus | scanning electron microscopy | - |
dc.subject.keywordPlus | skin culture | - |
dc.subject.keywordPlus | skin fibroblast | - |
dc.subject.keywordPlus | statistical analysis | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Cell Culture Techniques | - |
dc.subject.keywordPlus | Cell Proliferation | - |
dc.subject.keywordPlus | Cells, Cultured | - |
dc.subject.keywordPlus | Dermis | - |
dc.subject.keywordPlus | Epithelium | - |
dc.subject.keywordPlus | Female | - |
dc.subject.keywordPlus | Fibroblasts | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Keratinocytes | - |
dc.subject.keywordPlus | Lactic Acid | - |
dc.subject.keywordPlus | Mice | - |
dc.subject.keywordPlus | Mice, Inbred BALB C | - |
dc.subject.keywordPlus | Mice, Nude | - |
dc.subject.keywordPlus | Microspheres | - |
dc.subject.keywordPlus | Polyglycolic Acid | - |
dc.subject.keywordPlus | Regeneration | - |
dc.subject.keywordPlus | Skin Physiology | - |
dc.subject.keywordPlus | Skin Transplantation | - |
dc.subject.keywordPlus | Tissue Engineering | - |
dc.subject.keywordAuthor | tissue engineering | - |
dc.subject.keywordAuthor | poly(lactide-co-glycolide) microsphere | - |
dc.subject.keywordAuthor | skin regeneration | - |
dc.subject.keywordAuthor | keratinocyte | - |
dc.subject.keywordAuthor | dermal fibroblast | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.