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Transfection of mesenchymal stem cells with the FGF-2 gene improves their survival under hypoxic conditions
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Heesang Song | - |
dc.contributor.author | Kihwan Kwon | - |
dc.contributor.author | Soyeon Lim | - |
dc.contributor.author | Seok-Min Kang | - |
dc.contributor.author | Young-Guk Ko | - |
dc.contributor.author | ZhengZhe Xu | - |
dc.contributor.author | Ji Hyung Chung | - |
dc.contributor.author | Byung-Soo Kim | - |
dc.contributor.author | Hakbae Lee | - |
dc.contributor.author | Boyoung Joung | - |
dc.contributor.author | Sungha Park | - |
dc.contributor.author | Donghoon Choi | - |
dc.contributor.author | Yangsoo Jang | - |
dc.contributor.author | Nam-Sik Chung | - |
dc.contributor.author | Kyung-Jong Yoo | - |
dc.contributor.author | Ki-Chul Hwang | - |
dc.date.accessioned | 2024-06-13T02:22:45Z | - |
dc.date.available | 2024-06-13T02:22:45Z | - |
dc.date.created | 2018-06-20 | - |
dc.date.created | 2018-06-20 | - |
dc.date.issued | 2005-06 | - |
dc.identifier.citation | MOLECULES AND CELLS, Vol.19 No.3, pp.402-407 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204428 | - |
dc.description.abstract | Bone marrow mesenchymal stem cells (MSCs) have shown potential for cardiac repair following myocardial injury, but this approach is limited by their poor viability after transplantation. To reduce cell loss after transplantation, we introduced the fibroblast growth factor-2 (FGF-2) gene ex vivo before transplantation. The isolated MSCs produced colonies with a fibroblast-like morphology in 2 weeks; over 95% expressed CD71, and 28% expressed the cardiomyocyte-specific transcription factor, Nkx2.5, as well as (x-skeletal actin, Nkx2.5, and GATA4. In hypoxic culture, the FGF-2-transfected MSCs (FGF-2-MSCs) secreted increased levels of FGF-2 and displayed a threefold increase in viability, as well as increased expression of the anti-apoptotic gene, Bcl2, and reduced DNA laddering. They had functional adrenergic receptors, like cardiomyocytes, and exposure to norepinephrine led to phosphorylation of ERK1/2. Viable cells persisted 4 weeks after implantation of 5.0 x 10(5) FGF-2-MSCs into infarcted myocardia. Expression of cardiac troponin T (CTn T) and a voltage-gated Ca(2+) channel (CaV2.1) increased, and new blood vessels formed. These data suggest that genetic modification of MSCs before transplantation could be useful for treating myocardial infarction and end-stage cardiac failure. | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Transfection of mesenchymal stem cells with the FGF-2 gene improves their survival under hypoxic conditions | - |
dc.type | Article | - |
dc.citation.journaltitle | MOLECULES AND CELLS | - |
dc.identifier.wosid | 000230342100014 | - |
dc.identifier.scopusid | 2-s2.0-27244456956 | - |
dc.citation.endpage | 407 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 402 | - |
dc.citation.volume | 19 | - |
dc.identifier.kciid | ART001095761 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Byung-Soo Kim | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MARROW STROMAL CELLS | - |
dc.subject.keywordPlus | SKELETAL MYOBLAST TRANSPLANTATION | - |
dc.subject.keywordPlus | FIBROBLAST GROWTH FACTOR-2 | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | CARDIOMYOCYTES | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | MYOCARDIUM | - |
dc.subject.keywordPlus | HEARTS | - |
dc.subject.keywordAuthor | fibroblast growth factor-2 | - |
dc.subject.keywordAuthor | mesenchymal stem cell | - |
dc.subject.keywordAuthor | myocardial infarction | - |
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