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HEK 293 cell suspension culture using fibronectin-adsorbed polymer nanospheres in serum-free medium

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dc.contributor.authorJu Hee Ryu-
dc.contributor.authorSang‐Soo Kim-
dc.contributor.authorSeung‐Woo Cho-
dc.contributor.authorCha Yong Choi-
dc.contributor.authorByung‐Soo Kim-
dc.date.accessioned2024-06-13T02:23:29Z-
dc.date.available2024-06-13T02:23:29Z-
dc.date.created2018-06-20-
dc.date.created2018-06-20-
dc.date.issued2004-10-
dc.identifier.citationJournal of Biomedical Materials Research, Vol.71A No.1, pp.128-133-
dc.identifier.issn0021-9304-
dc.identifier.urihttps://hdl.handle.net/10371/204442-
dc.description.abstractPreviously, we reported on suspension culture of anchorage-dependent animal cells using plain polymer nanospheres in serum-containing medium. For commercial cell culture, it is more advantageous to use serum-free medium than serum-containing medium. To culture anchorage-dependent animal cells using polymer nanospheres in serum-free medium, the nanospheres need to be coated with cell adhesion proteins. In this study, we utilized fibronectin-adsorbed polymer nanospheres for suspension culture of anchorage-dependent animal cells in serum-free medium. Fibronectin was adsorbed onto poly(lactic-co-glycolic acid) nanospheres (433 nm in average diameter) by immersing the nanospheres in fetal bovine serum. The nanospheres were used to culture human embryonic kidney (HEK) 293 cells in serum-free medium in stirred suspension bioreactors. Nanospheres attached between HEK 293 cells and promoted cell aggregate formation compared with culture without nano spheres. Most cells in the aggregates were viable over a 10-day culture period. Importantly, the use of poly(lactic-co-glycolic acid) nanospheres promoted the cell growth significantly, compared with culture without nanospheres (3.8- vs 1.8-fold growth). The nanosphere culture method developed in this study removes the time-consuming and costly process of adaptation of anchorage-dependent animal cells to suspension culture in serum-free medium. This culture method may be useful for the large-scale suspension culture of various types of anchorage-dependent animal cells in serum-free medium. (C) 2004 Wiley Periodicals, Inc.-
dc.language영어-
dc.publisherHeterocorporation-
dc.titleHEK 293 cell suspension culture using fibronectin-adsorbed polymer nanospheres in serum-free medium-
dc.typeArticle-
dc.identifier.doi10.1002/jbm.a.30141-
dc.citation.journaltitleJournal of Biomedical Materials Research-
dc.identifier.wosid000223877000015-
dc.identifier.scopusid2-s2.0-4544230201-
dc.citation.endpage133-
dc.citation.number1-
dc.citation.startpage128-
dc.citation.volume71A-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorCha Yong Choi-
dc.contributor.affiliatedAuthorByung‐Soo Kim-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusAbsorption-
dc.subject.keywordPlusAdhesion-
dc.subject.keywordPlusBioreactors-
dc.subject.keywordPlusPolymers-
dc.subject.keywordPlusProteins-
dc.subject.keywordPlusAnimal cells-
dc.subject.keywordPlusCell growth-
dc.subject.keywordPlusPolymer nanospheres-
dc.subject.keywordPlusSerum-
dc.subject.keywordPlusCell culture-
dc.subject.keywordPlusfibronectin-
dc.subject.keywordPlusnanoparticle-
dc.subject.keywordPluspolyglactin-
dc.subject.keywordPluspolymer-
dc.subject.keywordPlusadsorption kinetics-
dc.subject.keywordPlusarticle-
dc.subject.keywordPlusbioreactor-
dc.subject.keywordPluscell adhesion-
dc.subject.keywordPluscell aggregation-
dc.subject.keywordPluscell culture-
dc.subject.keywordPluscell growth-
dc.subject.keywordPluscell suspension-
dc.subject.keywordPluscell viability-
dc.subject.keywordPluscontrolled study-
dc.subject.keywordPlusculture medium-
dc.subject.keywordPlusembryo-
dc.subject.keywordPlushuman-
dc.subject.keywordPlushuman cell-
dc.subject.keywordPlusimmersion-
dc.subject.keywordPluskidney cell-
dc.subject.keywordPlusmolecular dynamics-
dc.subject.keywordPlusmolecular mechanics-
dc.subject.keywordPlusstatistical significance-
dc.subject.keywordPlustechnique-
dc.subject.keywordPlusAnimals-
dc.subject.keywordPlusBioreactors-
dc.subject.keywordPlusCattle-
dc.subject.keywordPlusCell Aggregation-
dc.subject.keywordPlusCell Culture Techniques-
dc.subject.keywordPlusCell Line-
dc.subject.keywordPlusCulture Media, Serum-Free-
dc.subject.keywordPlusFibronectins-
dc.subject.keywordPlusHumans-
dc.subject.keywordPlusLactic Acid-
dc.subject.keywordPlusMaterials Testing-
dc.subject.keywordPlusNanotubes-
dc.subject.keywordPlusPolyglycolic Acid-
dc.subject.keywordPlusPolymers-
dc.subject.keywordAuthorsuspension culture-
dc.subject.keywordAuthornanosphere-
dc.subject.keywordAuthorserum-free medium-
dc.subject.keywordAuthoranchorage-dependent animal cell-
dc.subject.keywordAuthorhuman embryonic kidney 293 cell-
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  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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