Publications

Detailed Information

Tissue engineering of smooth muscle under a mechanically dynamic condition

Cited 24 time in Web of Science Cited 27 time in Scopus
Authors

Byung Soo Kim; Sung In Jeong; Seung Woo Cho; Janeta Nikolovski; David J. Mooney; Soo Hong Lee; O Ju Jeon; Tae Wan Kim; Sang Hyun Lim; Yoo Sun Hong; Choi, Cha Yong; Young Moo Lee; Soo Hyun Kim; Young Ha Kim

Issue Date
2003-12
Publisher
한국미생물·생명공학회
Citation
Journal of Microbiology and Biotechnology, Vol.13 No.6, pp.841-845
Abstract
In order for engineered tissues to find clinical utility, the engineered tissues must function appropriately. However, smooth muscle (SM) tissues engineered in vitro with a conventional tissue engineering technique may not exhibit contractile functions, because smooth muscle cells (SMCs) cultured in vitro typically revert from a contractile, differentiated phenotype to a synthetic, nondifferentiated phenotype and lose their ability to contract. SMCs in vivo typically reside in mechanically dynamic environments. We hypothesized that cyclic mechanical stretch induces the features of SMCs in in vitro engineered tissues to be similar to those of SMCs in native tissues. To test the hypothesis, aortic SMCs were seeded onto elastic, three-dimensional scaffolds and cultured in vitro under a cyclic mechanical stretching condition for 4 weeks. A significant cell alignment in a direction parallel to the cyclic stretching direction was found in the SM tissues exposed to cyclic stretching. The cellular alignment and alignment direction were consistent with those of native vascular SM tissues, in which SMCs in vivo align in the radial direction (parallel to stretching direction). In control tissues (SM tissues engineered without stretching), cells randomly aligned. The expression of SM alpha-actin and SM myosin heavy chain, phenotypic markers of SMCs in a contractile state, was upregulated in the stretched tissues by 2.5- and 2.0-fold, respectively, compared to SMCs in the control tissues. The cellular features of alignment and contractile phenotype of SMCs in the SM tissues engineered under a mechanically dynamic environment could allow the engineered SM tissues to exhibit contractile functions.
ISSN
1017-7825
URI
https://hdl.handle.net/10371/204451
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share