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Survival and function of rat hepatocytes cocultured with nonparenchymal cells or sinusoidal endothelial cells on biodegradable polymers under flow conditions

Cited 31 time in Web of Science Cited 41 time in Scopus
Authors

Satoshi Kaihara; Stephen Kim; Byung-Soo Kim; David J. Mooney; Koichi Tanaka; Joseph P. Vacanti

Issue Date
2000-09
Publisher
W B SAUNDERS CO
Citation
JOURNAL OF PEDIATRIC SURGERY, Vol.35 No.9, pp.1287-1290
Abstract
Background/Purpose: The authors have investigated hepatocyte transplantation using biodegradable polymer scaffolds as a possible treatment of end-stage liver disease. The purpose of this study was to investigate the survival rate and function of hepatocytes alone or cocultured with other cell types on 3-dimensional biodegradable polymers for 7 days under continuous flow conditions in vitro. Methods: Hepatocytes (group 1, n = 8), hepatocytes with nonparenchymal cells (group 2, n = 7), or hepatocytes with sinusoidal endothelial cells (group 3, n = 6) were isolated from Lewis rats and seeded onto the polymer scaffolds. The polymer devices subsequently were placed under continuous flow conditions for 7 days. Albumin production from the constructs was measured each day, and urea nitrogen synthesis was examined on day 7. The devices also were examined by histology at day 7. Results: Histology results showed the presence of numerous viable hepatocytes on polymer devices, with no differences in hepatocyte viability between the 3 groups. Albumin secretion in the culture medium gradually decreased by day 7. There also were no significant differences in albumin production or urea nitrogen synthesis between the 3 groups at day 7. Conclusions: Hepatocytes could survive on the 3-dimensional polymer scaffolds under flow conditions for 7 days, and albumin secretion and urea synthesis of hepatocytes were seen at day 7. Nonparenchymal cells acid sinusoidal endothelial cells had no measurable effect on hepatocyte function in our continuous flow culture system. J Pediatr Surg 35:1287-1290. Copyright (C) 2000 by W.B. Saunders Company.
ISSN
0022-3468
URI
https://hdl.handle.net/10371/204464
DOI
https://doi.org/10.1053/jpsu.2000.9298
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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