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Production of human hematopoietic progenitors in a clinical-scale stirred suspension bioreactor

DC Field Value Language
dc.contributor.authorKim, BS-
dc.date.accessioned2024-06-13T02:25:39Z-
dc.date.available2024-06-13T02:25:39Z-
dc.date.created2018-06-18-
dc.date.issued1998-06-
dc.identifier.citationBIOTECHNOLOGY LETTERS, Vol.20 No.6, pp.595-601-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://hdl.handle.net/10371/204483-
dc.description.abstractEx vivo expanded primitive hematopoietic cells can be utilized in bone marrow transplantation therapies to treat patients suffering from various cancers and hematopoietic malignancies. A high initial cell density (10(6) cells/mL) and the supplement of soluble factors secreted by stromal feeders in combination with growth-promoting (interleukin-3 and stem cell factor) and growth-inhibiting (macrophage-inflammatory protein-1 alpha) cytokines resulted in high, long-term expansions (17-fold over a 14-day culture period) of human hematopoietic progenitors in a stirred suspension bioreactor. This study demonstrated that a transplantable dosage of human hematopoietic progenitor cells (8.1 +/- 1.3 x 10(6) colony forming unit-granulocyte/macrophage) can be generated from approximately 10 mL of bone marrow aspirate in a 14-day culture using a 250 mL suspension bioreactor system.-
dc.language영어-
dc.publisherKLUWER ACADEMIC PUBL-
dc.titleProduction of human hematopoietic progenitors in a clinical-scale stirred suspension bioreactor-
dc.typeArticle-
dc.identifier.doi10.1023/A:1005362030908-
dc.citation.journaltitleBIOTECHNOLOGY LETTERS-
dc.identifier.wosid000074968500015-
dc.identifier.scopusid2-s2.0-0031872673-
dc.citation.endpage601-
dc.citation.number6-
dc.citation.startpage595-
dc.citation.volume20-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, BS-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusHUMAN BONE-MARROW-
dc.subject.keywordPlusSTEM-CELL FACTOR-
dc.subject.keywordPlusEX-VIVO-
dc.subject.keywordPlusCULTURES-
dc.subject.keywordPlusTRANSPLANTATION-
dc.subject.keywordPlusEXPANSION-
dc.subject.keywordPlusINTERLEUKIN-3-
dc.subject.keywordPlusINVITRO-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordPlusDIFFERENTIATION-
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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