MAMMALIAN CELL CULTIVATION ON SERUM-COATED MICROCARRIERS

Abstract

The cell culture on serum-coated microcarriers yielded higher efficiency of cell attachment to microcarriers and more favorable initial cell distribution on microcarriers than on the conventional microcarriers. By employing serum-coated microcarriers, the maximum cell density was increased by 46% m low serum medium and by 30% in 10% (v/v) serum-supplemented medium. Serum coating of microcarriers could provide cell attachment factors and may replace costly attachment factors supplemented in low serum medium and serum-free medium.